Longicollum biappendiculatum Zelski et al.
Index Fungorum number: IF560934
Sexual morph: Ascomata 545–585 ´ 155–160 µm, scattered, venter immersed in wood, with a hyaline, erumpent, central neck; venter 205–220 ´ 155–160 µm, membranous, subglobose, light brown. Neck long, 315–370 ´ 74–82 µm, cylindrical, hyaline, periphysate. Peridium 15–20 µm thick, composed of ca. 4–6 layers of pseudoparenchymatic cells; cells brown towards the outside, subhyaline and laterally compressed towards the inside. Paraphyses 140–170 µm long, 5–7 µm wide at the base, simple, septate, hyaline, broader at the base, tapering towards the apex. Asci 180–225 ´ 15–17 µm, (mean = 205 × 16 µm, n = 30), cylindrical, with a prominent J–, bi-partite apical ring, 2–4 ´ 3–5 µm wide, staining blue with aqueous nigrosin, with 8, overlapping uni-seriate ascospores, tapering towards the base, with a thin, elongate, tapering pedicel ca. 5 µm long, sometimes extending in length in water, separating from the hymenial layer, persistent. Ascospores 26–35 ´ 12–14 µm, (mean = 28 ´ 13 µm, n = 50), broadly ellipsoidal, hyaline, multi-guttulate, non-septate, with short hyaline, bipolar, ephemeral, mucilaginous appendages, ca. 3–6 ´ 6–9 µm. Colonies on PYG + Ab agar immersed, brown, comprised of hyaline to light brown septate, branching hyphae. Anamorphic state not observed. Asexual morph: Undetermined.
Specimens examined: BRAZIL, Bahia, stream at Serra da Jiboia, on submerged bark debris; COSTA RICA, Heredia, La Selva Biological Station, La Selva stream, on submerged bark debris; PERU, Camanti, stream at Quincemil Trail 1, on submerged woody debris; USA, Florida, Wildcat Lake, Ocala National Forest, on submerged woody debris (Zelski et al.2011).
Fig.1. Longicollum biappendiculatum (ILL 40794, holotype). No. 1. Whole mount of ascoma showing long hyaline neck and light brown venter. No. 2. Longitudinal section through ascoma. No. 3. Periphysate neck. No. 4. Longitudinal section through peridium. No. 5. Paraphyses. Scale bars: 1–2 = 100 µm, 3–5 = 20 µm. (photo grabbed from Zelski et. Al 2011)
Fig.2. Longicollum biappendiculatum (ILL 40794, holotype). No. 6. Mature and young asci (arrow). No. 7. Mature ascus showing overlapping uni-seriate hyaline ascospores. No. 8. Mature expanded ascus showing pedicel with tiny extension (inset). No. 9. Mature ascus apex with apical ring. No. 10. Bi-partite apical ring (arrows). No. 11–13. Mature ascospores showing bipolar mucilaginous appendages (arrows). Scale bars: 6–13 = 20 µm. (photo grabbed from Zelski et. Al 2011)