Kirschsteiniothelia rostrata J. Yang & K.D. Hyde
Index Fungorum number: IF552909; Facesoffungi number: FoF 03183
Etymology: referring to the rostrate conidia.
Holotype: MFLU 15-1154
Saprobic on decaying plant substrates. Asexual morph Colonies effuse, black, hairy. Mycelium partly immersed, partly superficial in the substrate. Conidiophores macronematous, mononematous, solitary or sometimes caespitose, cylindrical, wide at the base, tapering towards the apex, straight or slightly flexuous, smooth, dark brown, slightly swollen at the base, 7–24 septate, 190–450 × 9–15 µm (= 280 × 12 µm, n = 20). Conidiogenous cells monoblastic, integrated, terminal, determinate, cylindrical or lageniform, smooth, mid to dark brown. Conidia acrogenous, solitary, dry, olivaceous brown to mid-brown, pale at the apex, obclavate, rostrate, smooth, straight or curved, 8–13-euseptate, sometimes with a mucilaginous sheath, 80–150 × 10–20 µm ( = 115 × 15 µm, n = 30); rounded and 3–7 μm wide at the apex; truncate and 3–7 μm wide at the base. Sexual morph Undetermined. (Descriptions based on Hyde et al. 2017)
Culture characteristics: Conidia germinating on PDA within 24 h Germ tubes produced from both ends. Colonies on PDA reaching 5-10 mm diameter after one week at 25 °C, in natural light, circular, dark green to black, with dense, fluffy aerial mycelium on the surface with sparse mycelium at the entire margin, black in reverse.
Material examined: THAILAND, Prachuap Khiri Khan Province, on decaying wood submerged in a freshwater stream, 25 December 2014, Jaap van Strien, Site 4-23-2 (MFLU 15-1154 holotype); ibid., (GZAAS 17-0012, isotype), ex-type living cultures MFLUCC 15-0619, GZCC 15-0061.
GenBank numbers ITS:KY697280; LSU:KY697276; SSU:KY697278; TEF1:MF953397
Notes: There are a few molecular data of Kirschsteiniothelia compared to morphological studies (Boonmee et al. 2012; Hyde et al. 2013). It is problematic to link this asexual morph with the sexual morph that lacks molecular data and without known asexual morph. Here we propose to introduce this asexual fungus as a new species based on both morphology and phylogenetic analyses. Kirschsteiniothelia rostrata resembles K. tectonae Doilom, D.J. Bhat & K.D. Hyde in having obclavate, rostrate conidia with mucilaginous sheaths and truncate at the apex of conidiophores. However, conidiophores of Kirschsteiniothelia rostrata (190–450 × 9–15 µm) are much longer than those of K. tectonae (up to 200 µm long, 4–8 µm wide). Kirschsteiniothelia aethiops, K. emarceis S. Boonmee & K.D Hyde, K. lignicola S. Boonmee & K.D. Hyde and K. submersa H.Y. Su & K.D. Hyde are distinguished from the new taxon in having clavate conidia and rounded apex of conidiophores. The combined LSU, SSU and ITS phylogenetic analysis shows that Kirschsteiniothelia rostrata represents a sister taxon to K. tectonae with good separation. (Notes taken from Hyde et al. 2017)
Boonmee S, KoKo TW, Chukeatirote E, Hyde KD et al (2012) Two new Kirschsteiniothelia species with Dendryphiopsis anamorphs cluster in Kirschsteiniotheliaceae fam. nov. Mycologia 104:698–714
Hyde KD, Jones EBG, Liu JK, Ariyawansa H et al (2013) Families of Dothideomycetes. Fungal Divers 63:1–313.
Hyde KD, Norphanphoun C, Abreu VP, et al (2017) Fungal diversity notes 603–708: taxonomic and phylogenetic notes on genera and species. Fungal Divers 87:1–235. https://doi.org/10.1007/s13225-017-0391-3
Fig 1. Kirschsteiniothelia rostrata (MFLU 15-1154 holotype). a, c–d. Fruiting body. b, e–f. Conidiophores. g–h. Conidiogenous cells. i–q. Conidia. r–s. Germinated conidia on PDA. t–u. Culture. t from above. u from below. Scale bars a, s = 100 μm, b–e = 80 μm, f–h = 30 μm, i–q = 40 μm, r = 50 μm.