Index Fungorum number: IF555646 Facesoffungi number: FoF05418
Sexual morph: Ascomata immersed with neck erumpent through host surface, globose to subglobose, dark brown to black, solitary. Peridium composed of an inner layer of flattened hyaline cells and an outer layer of irregular, heavily pigmented, thick-walled, pale-brown to dark-brown cells of textura angularis. Paraphyses hyaline, septate, unbranched. Asci 8-spored, unitunicate, cylindrical to clavate, hyaline. Ascospores filiform, aseptate, guttulate, hyaline, smooth-walled. Asexual morph: Undetermined.
Notes: Aquafiliformis morphologically resembles Ceratosphaeria in having globose to subglobose, dark brown to black ascomata, cylindrical, 8-spored, unitunicate asci and filiform, hyaline, guttulate ascospores (Huhndorf et al. 2008). However, the phylogenetic analysis of combined LSU, SSU, RPB2 and TEF1a sequence data show that our newly obtained strains (MFLUCC 16–1341, MFLUCC 18–1338) clusters in Magnaporthaceae, while Ceratosphaeria belongs to the newly introduced Ceratosphaeriaceae. Wijayawardene et al. (2018) accepted 23 genera in the family Magnaporthaceae. Twenty genera with available molecular sequence data are included in our phylogenetic analysis and 17 of them clusters together, while the newly obtained strains MFLUCC 16–1341 and MFLUCC 18–1338 form a separate clade in Magnaporthaceae and close to Muraeriata (SMH 2008a, SMH 2008b). However, our strain differs from Muraeriata species in having globose to subglobose ascomata, a peridium composed of an inner layer of flattened hyaline cells and an outer layer of irregular, heavily pigmented, thick-walled, pale brown to dark brown cells of textura angularis and filiform, aseptate ascospores, while Muraeriata species have lageniform to globose ascomata, a peridium with a middle layer of large, polygonal to irregular, hyaline cells that collapse or rupture creating large empty pockets, with an external brown crust and narrowly fusiform, septate ascospores (Huhndorf et al. 2008). The genera Clavatisporella and Herbampulla were also placed in Magnaporthaceae, but the sequence data for these two genera are unavailable, and morphology of our fungus are different from those of Clavatisporella and Herbampulla by its filiform ascospores (Scheuer and Nograsek1993; Hyde 1995a). Therefore, we introduce a new genus Aquafiliformis to accommodate our collections
Type species: Aquafiliformis lignicola Z.L. Luo, K.D. Hyde & H.Y. Su