Aquafiliformis lignicola Z.L. Luo, K.D. Hyde & H.Y. Su
Index Fungorum number: 555647; Facesoffungi number: FoF05418
Sexual morph: Ascomata 275–335 µm high, 300–356 µm diam., immersed with erumpent neck through host surface, globose to subglobose, dark brown to black, solitary. Peridium 24–34 µm thick, composed of an inner layer of flattened hyaline cells and an outer layer of irregular, heavily pigmented, thick-walled, pale brown to dark brown cells of textura angularis. Paraphyses 4.5–7.5 µm wide, hyaline, septate, unbranched. Asci 76–86 ´ 8–12 µm (x̄ = 81 ´ 10 µm, n = 25), 8-spored, unitunicate, cylindrical to clavate, hyaline. Ascospores 57–69 ´ 2.5–3.5 µm (x̄ = 63 ´ 3 µm, n = 20), filiform, guttulate, aseptate, hyaline, smooth-walled. Asexual morph: Undetermined.
Specimen examined: CHINA, Yunnan Province, saprobic on decaying wood submerged in a freshwater River, April 2015, X.C. Tao, S-478 (MFLU 18–2325, holotype; HKAS 92814, isotype), ex-type living culture MFLUCC 16–1341; Ibid., saprobic on decaying wood submerged in a freshwater stream in Cangshan Mountain, August 2016, H.W. Shen, S-717 (MFLU 18–2324, paratype), living culture MFLUCC 18–1338.
Notes: Aquafiliformis lignicola resembles Neogaeumannomyces bambusicola in having immersed, globose to subglobose, solitary ascomata, unitunicate, cylindrical, hyaline asci and filiform, hyaline, guttulate ascospores (Liu et al.2015a, b). However, Aquafiliformis lignicola differs from Neogaeumannomyces bambusicola in having different sizes of ascomata, asci and ascospores. Aquafiliformis lignicola has aseptate ascospores, while Neogaeumannomyces bambusicola has 2–3-septate ascospores. Phylogenetic analysis also support that they belong to different genera.
Fig. Aquafiliformis lignicola (MFLU 18–2325, holotype) a. Appearance of necks on substrate. b. Section through ascoma. c. Structure of peridium. d. Paraphyses. e, f. Asci. g–j. Ascospores. Culture on PDA from surface (k) and reverse (l). Scale bars: b = 150 µm, c = 50 µm, d– f = 30 µm, g –j = 20 µm. (photo grabbed from Z.L. Luo et.al 2019)