Pleosporales » Lindgomycetaceae » Lindgomyces

Lindgomyces cigarosporus

Lindgomyces cigarosporus Raja, J. Fourn., Paguigan & Oberlies [as ‘cigarospora’]

 

Index Fungorum number: IF821195

Holotype: ILLS80482

Etymology: from Latin cigaro = cigar, in reference to the cigar-shaped ascospores.

 

Saprobic on decaying wood. Sexual morph: Ascomata 280–290× 330–340 μm, black, partially superficial to immersed, gregarious, globose to subglobose, ostiolate, short papillate. Peridium composed of two layers: inner layer composed of small, pale brown, isodiametric cells, textura angularis in surface view, outer layer of pigmented, thick-walled, pseudoparenchymatic cells. Pseudoparaphyses cellular, abundant, 1–2 μm wide, septate, slightly constricted at the septa, anastomosing above the asci. Asci 112–162×21– 32 μm (mean and SD = 131 ± 12 × 26 ± 3 μm, n = 25), broadly clavate, thick-walled, and rounded at the apex, with an apical chamber, fissitunicate, tapering to a long stipe at the base, 10–15 μm, eight-spored. Ascospores 44–60 (−70) × 7– 10 (−13) μm (mean and SD = 50 ± 4 × 8 ± 1 μm, n = 40), overlapping biseriate to triseriate, fusiform to cylindrical with rounded ends, hyaline, one-septate; primary septum supra median to mostly median (0.46–0.50; average 0.5, n=25), with 2–3 large lipid guttules in each cell, with short, rounded terminal ephemeral appendages, visible when ascospores are mounted in glycerin and/or lactic acid. Asexual morph: Undetermined (Description from Raja et al. 2017)

 

Specimens examined: USA, North Carolina, 35°9.215′N, 81°1.231′W, Lake Wylie, on submerged wood, 30 August 2014, Huzefa A. Raja and Vincent P. Sica, G619 (ILLS80482), ex-type living culture DSM 103500, single ascospore isolate from holotype. (Notes from Raja et al. 2017)

 

Freshwater distribution: North Carolina (Raja et al. 2017)

 

Notes: Morphologically, L. cigarospora is comparable with L. rotundatus, but the ascospores of L. cigarospora are fusiform to cylindrical with rounded ends, equipped with short, rounded terminal, ephemeral appendages, while those of L. rotundatus are cylindrical with rounded ends and surrounded by fusiform gelatinous sheath. There is no expanding fusiform gelatinous sheath in L. cigarospora, but ascospores of L. rotundatus are equipped with a sheath, 4 μm thick at sides, and 30–35 μm long at both ends, expanding to form appendages up to 200 μm long (Hirayama et al. 2010). The morphological data are further corroborated with molecular data, which includes the (SSU+LSU nrDNA) and nuclear ribosomal ITS data, clearly indicating that L. cigarospora is a phylogenetically distinct species (Raja et al. 2017).

 

Fig. 1 Lindgomyces cigarospora (from HOLOTYPE, ILLS 80482). a Ascomata on balsa wood in culture from ex-type culture (DSM 103500, G619; ascomata produced in culture are sterile). b Asci with interascal pseudoparaphyses. c–e Asci; note bitunicate ascus in d. f–i Ascospores; note arrows in g showing short, rounded terminal ephemeral appendages on ascospores mounted in glycerin and lactic acid. Scale bars: a = 500 μm; b, c, e = 50 μm; d, f, g, h = 20 μm; i = 5 μm (Raja et al. 2017; http://libres.uncg.edu/ir/uncg/f/N_Oberlies_Additions_2017.pdf).

 

References

Raja HA, Paguigan ND, Fournier J, Oberlies NH (2017) Additions to Lindgomyces (Lindgomycetaceae, Pleosporales, Dothideomycetes), including two new species occurring on submerged wood from North Carolina, USA, with notes on secondary metabolite profiles. Mycol Prog 16:535–552. https://doi.org/10.1007/s11557-017-1282-4

Hirayama K, Tanaka K, Raja HA, Miller AN, Shearer CA (2010) A molecular phylogenetic assessment of Massarina ingoldiana sensu lato. Mycologia 102:729–746. doi:10.3852/09-230

 

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