Pleosporales » Lindgomycetaceae » Lindgomyces

Lindgomyces cinctosporus

Lindgomyces cinctosporus Raja, A.N. Mill. & Shearer [as ‘cinctosporae’]

 

Index Fungorum number: IF584394

Holotype: ILL40791

Etymology: cincto = Latin meaning surround/enclosed referring to the ascospore, which is surrounded by a gelatinous sheath

 

Sexual morph: Ascomata on wood 285-330 X 374-426 um, subglobose to conical, black, appearing as raised dome like structures on substrate, solitary to gregarious, immersed to partly immersed, ostiolate, papillate, clypeate. Beak 40–70 × 85–90 um, central, conical to cylindrical, aperiphysate. Ascomal wall 45–68 um wide, in longitudinal section composed of an inner layer of hyaline, elongate, angular, thin-walled cells 5–7 × 3–5 um, and an outer layer of angular cells occluded with brown amorphous material associated with decomposed wood. Pseudoparaphyses numerous, sheet-like, septate, constricted at the septa, 1.5–3 um wide, anastomosing. Asci 146–198 × 22–35 um ( = 167 × 29 um, n = 15), fissitunicate, clavate, broadly rounded at the apex, tapering to a short stipe, thick-walled, with or without an apical chamber. Ascospores 40–58 × 10–18 um ( = 50 ×14 um, n = 50), overlapping biseriate, oblong-fusiform, slightly rounded at the apices, hyaline, one-septate when young, with the primary septum almost median (0.46–0.57; = 0.50, n = 50), becoming three-septate and brown with age, with one large and one small guttule in each cell, occasionally filled with numerous small, lipid droplets; upper cell broader than the lower cell; surrounded by a gelatinous sheath ca. 2 um wide along the side of the ascospores and 3–4 um long at the ascospore apices, staining in aqueous nigrosin; in water sheath becoming amorphous and spreading to ca. 8–12 um wide. Colonies on PYG immersed with a dense mat of aerial hyphae; immersed hyphae brown black. Asexual morph: Unknown. (Description from Hirayama et al. 2010)

 

Specimens examined: USA. NORTH CAROLINA, Deep Creek, 35o28'23"N, 83o25'39"W, water 21oC, pH 5, 16 Jul 2000, Jinx Campbell, A430-1; NORTH CAROLINA, Cheoah Lake near Little Tennessee River, 35o27'06"N, 83o49'25"W, on submerged wood, water 28 oC, pH 4.5, 19 Jul 2000, Jinx Campbell, A430-3. TENNESSEE: Great Smoky Mountains National Park, Green Brier Area, 35o45'054"N, 83o06'530"W, water 22 C, pH 5, on submerged wood, 2 Jul 2002, HAR f Nate Hamburger, R56-1 (ILL 40791 HOLOTYPUS designated here; culture Raja R56-1); Great Smoky Mountains National Park, Forge Creek, 35o33'45"N, 83o50'45"W, water 19oC, 6 Sep 2005, HAR f ANM, R56-2; Great Smoky Mountains National Park, Fletcher Creek, on road to Cosby campground, 35o46'52.895"N, 83o13"0.264"W, on submerged wood, pH 5-5.5, 28 Jul 2008, HAR, ANM, Alberto M. Stchigel & Misericordia Calduch, R56-3;

 

Freshwater distribution: USA (Hirayama et al. 2010)

 

Notes: Lindgomyces cinctosporae differs from other species of Lindgomyces in the relatively wide ascospores and a surrounding ruffled gelatinous sheath. The sheath eventually becomes amorphous and enlarged in water but not nearly to the size of the sheaths of the other Lindgomyces species. The ascospores of L. cinctosporae are oblong-fusiform and slightly rounded at the apices, whereas those of L. ingoldianus are fusiform with acute ends and those of L. rotundatus are somewhat cylindrical with broadly rounded apices before fixation. Thus far L. cinctosporae has been reported only from submerged wood collected in Great Smoky Mountains National Park. Lindgomyces cinctosporae (isolate R56-1) was tested for the production of extracellular enzymes in vitro and was found positive for cellulase, endoglucanase, beta-glucosidase, xyla. (Notes from Hirayama et al. 2010)

 

Figs. 26–36. Lindgomyces cinctosporae. 26. Ascomata on wood. 27. Longitudinal section through the ascoma (note dark clypeus around the ostiole). 28. Cellular pseudoparaphyses. 29, 30. Clavate, thick-walled asci. 31. Fissitunicate ascus with endoascus extending from ectoascus. 32. Ascospores with a gelatinous sheath mounted in water. 33. Ascospores stained with India ink showing gelatinous sheath. 34. Ascospores stained with aqueous nigrosin showing expanding gelatinous sheaths. 35. Ascospore sheath in glycerin. 36. Mature, three-septate ascospores beginning to germinate. Bars: 26 = 1 um; 27 = 50 um; 28, 30, 33–36 = 10 um; 29, 31, 32 = 20 um. (Hirayama et al. 2010; http://fungi.life.illinois.edu/articles/Hirayama%20et%20al.%202010.pdf)

 

References

Hirayama K, Tanaka K, Raja HA, Miller AN, Shearer CA (2010) A molecular phylogenetic assessment of Massarina ingoldiana sensu lato. Mycologia 102:729–746. https://doi.org/10.3852/09-230

Raja HA, Paguigan ND, Fournier J, Oberlies NH (2017) Additions to Lindgomyces (Lindgomycetaceae, Pleosporales, Dothideomycetes), including two new species occurring on submerged wood from North Carolina, USA, with notes on secondary metabolite profiles. Mycol Prog 16:535–552. https://doi.org/10.1007/s11557-017-1282-4

 

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