Lindgomyces ingoldianus
Lindgomyces ingoldianus (Shearer & K.D. Hyde) K. Hiray., Kaz. Tanaka & Shearer
Basionym: Massarina ingoldiana Shearer & K.D. Hyde, Mycologia 89(1): 114 (1997)
Index Fungorum number: IF515189
Holotype: ILLS 52289
Sexual morph: Ascomata 200-260 µm high, 340-450 µm wide, scattered, immersed to erumpent, subglobose, black, stiolate. Ostiole central, rounded. Beak 45–70 µm long, 90–140 µm wide, short-papillate, composed of subglobose brown cells. Ascomal wall 20–35 µm thick, composed of an inner layer of polygonal to sub globose hyaline, thin-walled cells 7–12 × 2.5–5 µm, and an outer layer of small subglobose, brown cells 2–4 × 2–4 µm, sometimes poorly developed at the base. Pseudoparaphyses cellular, numerous, 1.5–3 µm wide, hyaline, anastomosing, branched, covered with gelatinous material. Asci (110–)120–167.5(–182.5) × 25–32.5 µm (x̄ = 148.0 × 27.9 µm, n = 50), fissitunicate, cylindrical to cl vate, rounded at the apex, with an apical chamber, with eight overlapping biseriate to triseriate ascospores. Ascospores (47–) 50–59 × 9–11(–12) µm (x̄ = 55.3 × 9.9 µm, n = 50), L/W (4.7–) 4.9–6.6 (x̄ = 5.6, n = 50), fusiform with acute ends, straight or slightly curved, with the primary septum almost median (0.47–0.54; x̄ = 0.50, n = 50), filled with small lipid droplets that have a foamy appearance, slightly constricted at primary septum, with a broad upper cell, hyaline, smooth, becoming three septate and pale brown with age, surrounded by a gelatinous sheath. Sheath about 1 µm thick at sides, 20–30 µm long at each end. Sheath expands at both ends to form large, sticky appendages up to 240 µm long. Asexual morph: Unknown. (Description from Hirayama et al. 2010)
Freshwater distribution: Australia (Shearer and Hyde 1997), Brunei (Shearer and Hyde 1997), China (Tsui et al. 2000), Japan (Hirayama et al. 2010), Malaysia (Hyde and Aptroot 1998b), Thailand (Sivichai et al. 2002), USA (Shearer and Hyde 1997)
Specimens examined: USA. WISCONSIN: Adams County, Lemonweir River, 43o46'16"N, 89o53'10"W, on submerged decorticated woody debris, 31 Jul 1992, CAS and JLC A-39-1 (HOLOTYPUS ILLS 52289; culture ATCC 200398). JAPAN. OKINAWA: Iriomote, Oomijya River, 24°23'695"N, 123°51'781"E, on submerged decorticated woody debris, 28 Sep 2007, KH 100 (HHUF 30006; culture JCM 16479).
Notes: This species is almost identical to L. rotundatus (see below) in terms of overall morphology and lignicolous substrates in aquatic habitats but can be distinguished from the latter by relatively larger ascospores with acute ends. (Notes from Hirayama et al. 2010)
Figs. 1-9. Lindgomyces ingoldianus. 1, 2. From type specimen, ILLS. 1. Median section through ascoma, X 325. 2. Median section through an ascoma showing darkened clypeus-like region around wall of beak, X 335. 3. From specimen A-140-2. Ascospore with fusiform sheath (arrow) being discharged from endoascus, X 440. 4. From specimen HKU(M) 1552. Partial section through an ascoma, X 660. 5. From specimen A-140-2. Asci and paraphyses from fresh material, X 304. 6. From specimen HKU (M) 1552. Asci showing thick wall, X 450. 7, 8. From specimen A-140-2. 7. Endoascus emerging from ectoascus, X 315. 8. Ascospore showing foamy appearance of ascospore and unexpanded ascospore sheath (arrow), X 390. 9. From specimen HKU(M). Guttulate ascospore in water; sheath is no longer visible, X 720. (Shearer and Hyde 1997; https://hub.hku.hk/bitstream/10722/53364/1/33393.pdf)
Figs. 101-13. Lindgomyces ingoldianus. 10-12. From type specimen, ILLS. 10, 11. Material has been mounted in water, stained with India ink, and then rinsed with water. 10. Ascospore surrounded by a gelatinous sheath, X 860. 11. Sticky sheaths of several ascospores adhering to an air bubble, X 150. 12. Ascospore with sheath immersed in India ink, X 180. 13. From specimen A-140-2. Ascospore with sheath immersed in India ink, X 135. (Shearer and Hyde 1997; https://hub.hku.hk/bitstream/10722/53364/1/33393.pdf)
Figs. 2–13. Lindgomyces ingoldianus. 2. Ascospore with a sheath (in India ink). 3–6. Ascospores. 7. Cellular pseudoparaphyses. 8, 9. Asci. 10, 11. Ascomata on host surface. 12. Ascoma in longitudinal section. 13. Ascomal wall. (2, 5 from culture ATCC 200398; 3, 4, 7, 9–13 from ILLS 52289 holotype; 6, 8 from HHUF 30006). Bars: 2, 12 = 50 µm; 3–7 = 10 µm; 8, 9 = 30 µm; 10 = 250 µm; 11 = 125 µm; 13 = 20 µm. (Hirayama et al. 2010; http://fungi.life.illinois.edu/articles/Hirayama%20et%20al.%202010.pdf)
References
Tsui CKM, Hyde KD, Hodgkiss IJ (2000) Biodiversity of fungi on submerged wood in Hong Kong streams. Aquat Microb Ecol 21:289–298. https://doi.org/10.3354/ame021289
Hirayama K, Tanaka K, Raja HA, Miller AN, Shearer CA (2010) A molecular phylogenetic assessment of Massarina ingoldiana sensu lato. Mycologia 102:729–746. https://doi.org/10.3852/09-230
Sivichai S, Jones EBG, Hywel-Jones N (2002) Fungal colonisation of wood in a freshwater stream at Tad Ta Phu, Khao Yai National Park, Thailand. Fungal Divers 10:113–129
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