Lindgomyces madisonensis
Lindgomyces madisonensis Raja & Oberlies
Index Fungorum number: IF812940
Holotype: ILLS 73408
Etymology: Name refers to ‘Madison’ town in North Carolina where the type species was collected.
Sexual morph: Ascomata on wood 248–276 × 295–326 µm, black, superficial to partially immersed, scattered, globose to subglobose, ostiolate, short papillate; papilla 37 × 47 µm. Peridium c. 25–35 µm wide, composed of dark irregularly shaped cells. Pseudoparaphyses cellular, abundant, c. 2 µm wide, covered with gelatinous material, septate, anastomosing above the asci. Asci 100–157 × 14–16 µm (mean and SD = 125 ± 16 × 15 ± 1 µm, n = 20), clavate to cylindrical, narrow at the apex, fissitunicate, tapering to a short stipe at the base, with eight overlapping biseriate ascospores at ascus apex becoming uniseriate at ascus base. Ascospores 36–43 × 6–9 µm (mean and SD = 39 ± 2 × 7 ± 1 µm, n = 45), fusiform, straight or slightly curved, tapering at the apices, 1-septate, constricted at the septum; primary septum supra-median to mostly median (0.43–0.5; average 0.5, n = 40), hyaline when young; ascospores become 3-septate and brown with age, multiguttulate, equipped with short bipolar appendages, c. 2 µm long; appendages ephemeral in water and not clearly visible in glycerin and lactic acid. Asexual morph: Undetermined. (Description from Crous et al. 2015)
Material examined: A specimen derived from a culture isolated from submerged decorticated wood and grown on alfalfa (Medicago sp.) stems. usA, North Carolina, Rockingham County, Big Beaver Island Creek, Madison, N36°27'40.0" W80°01'46.0", water 10 °C, pH 5, 26 Apr. 2013, Huzefa A. Raja & Nicholas H. Oberlies, G416a (holotype ILLS 73408, ex-type culture DSM 100629 = CBS 140367, single ascospore isolate from holotype.
Freshwater distribution: USA (Crous et al. 2015)
Notes: Morphological features of this species, such as globose to subglobose, scattered, ostiolate and papillate ascomata; numerous cellular pseudoparaphyses; 8-spored, bitunicate, fissitunicate, cylindrical asci with short, furcate pedicel; narrowly fusiform, hyaline, 1-septate ascospores bearing bipolar mucilaginous appendages, becoming brown and 3-septate with age, agree with the generic concept of the recently circumscribed genus Lindgomyces (Hirayama et al. 2010). Lindgomyces currently includes eight species, L. ingoldianus (type species), L. angustiascus, L. apiculatus, L. breviappendiculatus, L. cinctosporus, L. griseosporus, L. lemonweirensis and L. rotundatus (Hirayama et al. 2010, Raja et al. 2011, 2013, Zhang et al. 2014). All species of Lindgomyces described thus far have been reported from submerged wood in freshwater habitats. Lindgomyces madisonensis is morphologically most similar to L. apiculatus in having biapiculate gelatinous appendages. The former, however, differs from the latter in having narrow asci (100–157 × 14–16 µm in L. madisonensis vs 85–125 × 17–25 µm in L. apiculatus) and ascospores (36–43 × 6–9 µm in L. madisonensis vs 33–43 × 8–11 µm in L. apiculatus). In addition, molecular phylogenetic analyses of combined SSU and LSU as well as ITS clearly separate the two species. Other species in the genus that have biapiculate appendages include L. biappendiculatus and L. angustiascus. Lindgomyces madisonensis differs from these taxa in both morphology and size of asci and ascospores. Molecular phylogenetic analysis also clearly distinguishes the aforementioned biapiculate spp. Raja et al. (2011) provided a key to six species of Lindgomyces described previously. (Notes from Crous et al. 2015)
Fig 1. Lindgomyces madisonensis. Arrows on apical apices of ascospores show biapiculate appendages; arrow on ascus tip showing gelatinous material. Photos: Huzefa A. Raja. Scale bars: ascoma = 200 µm, all others = 20 µm. (Crous et al. 2015; https://www.fungalplanet.org/content/pdf-files/FungalPlanet390.pdf)
References
Crous PW, Wingfield MJ, Le Roux JJ, Richardson DM, Strasberg D, Shivas RG, Alvarado P, Edwards J, Moreno G, Sharma R, Sonawane MS, Tan YP, Altés A, Barasubiye T, Barnes CW, Blanchette RA, Boertmann D, Bogo A, Carlavilla JR, Cheewangkoon R, Daniel R, De Beer ZW, De Jesús Yáñez-Morales M, Duong TA, Fernández-Vicente J, Geering ADW, Guest DI, Held BW, Heykoop M, Hubka V, Ismail AM, Kajale SC, Khemmuk W, Kolařík M, Kurli R, Lebeuf R, André Lévesque C, Lombard L, Magista D, Manjón JL, Marincowitz S, Mohedano JM, Nováková A, Oberlies NH, Otto EC, Paguigan ND, Pascoe IG, Pérez-Butrón JL, Perrone G, Rahi P, Raja HA, Rintoul T, Sanhueza RMV, Scarlett K, Shouche YS, Shuttleworth LA, Taylor PWJ, Greg Thorn R, Vawdrey LL, Solano-Vidal R, Voitk A, Wong PTW, Wood AR, Zamora JC, Groenewald JZ (2015) Fungal planet description sheets: 371–399. Persoonia Mol Phylogeny Evol Fungi 35:264–327. https://doi.org/10.3767/003158515X690269
Hirayama K, Tanaka K, Raja HA, Miller AN, Shearer CA (2010) A molecular phylogenetic assessment of Massarina ingoldiana sensu lato. Mycologia 102:729–746. https://doi.org/10.3852/09-230
Raja HA, Tanaka K, Hirayama K, Miller AN, Shearer CA (2011) Freshwater ascomycetes: Two new species of Lindgomyces (Lindgomycetaceae, Pleosporales, Dothideomycetes) from Japan and USA. Mycologia 103:1421–1432. https://doi.org/10.3852/11-077
Raja HA, Tanaka K, Hirayama K, Miller AN, Shearer CA (2011) Freshwater ascomycetes: Two new species of Lindgomyces (Lindgomycetaceae, Pleosporales, Dothideomycetes) from Japan and USA. Mycologia 103:1421–1432. https://doi.org/10.3852/11-077
Zhang Y, Zhang X, Fournier J, Chen J, Hyde KD (2014) Lindgomyces griseosporus, a new aquatic ascomycete from Europe including new records. Mycoscience 55:43–48. https://doi.org/10.1016/j.myc.2013.05.003
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