Pleosporales » Lindgomycetaceae » Lolia

Lolia dictyospora

Lolia dictyospora Abdel-Aziz

Index Fungorum number: IF551996; Facesoffungi number: FoF 02013

Holotype: CBS H-22131

Etymology: From the dictyospores of the fungus.

 

Saprobic on submerged decayed rachis of the Phoenix dactylifera L. Sexual morph: Ascomata 180–340 μm diam., solitary, immersed to erumpent, globose to subglobose with flattened base, papillate, ostiolate, dark-brown to black, coriaceous to sub-carbonaceous. Peridium 27–56 μm wide at the base and sides, forming textura angularis, two layered; outer layer 12–30 μm wide, consists of polygonal dark-brown cells with melanized thick-walls; inner layer 15–26 μm wide, consists of polygonal flattened cells, thin-walled, hyaline to yellow-brown cells. Pseudoparaphyses 0.5–1 μm wide, thin, numerous, branched, embedded in gel, anastomosing above asci and emerged into the ostiolar canal. Asci 90–124 × 12–15 μm (x̅ = 109.2 × 13.4 μm, n = 15), four, six to eight-spored, clavate, bitunicate, fissitunicate, short pedicellate, with ocular chamber. Ascospores 14–19 × 4–7 μm (x̅ = 17.1 × 5.6 μm, n = 50), 3–6 transverse septa, constricted at the septa, 0–1 longitudinal septum, occasionally with oblique septa, yellow-brown to dark-brown in color, guttulate, clavate in shape, apical cell is the widest, surrounded by prominent gelatinous sheath, 2–8 μm thick, sometimes swollen in water to form globular gelatinous structures 10–15 μm diam. Asexual morph: Undetermined. (Descriptions from Abdel-Aziz 2016)

 

Material examined: EGYPT, Sohag, River Nile, on submerged decayed rachis of Phoenix dactylifera (Arecaceae), 14 August 2012, F.A. Abdel-Aziz (CBS H-22131, holotype); ex-type living culture, MD1313.

 

Notes: Lolia dictyospora is different from L. aquatica in having smaller ascomata (180–340 μm vs. 500–800 μm for L. dictyospora and L. aquatica respectively), smaller 4 to 8-spored asci (90–124 × 12–15 μm vs. 109–145 × 17–26 μm for L. dictyospora and L. aquatica respectively) and smaller dictyospores (14–19 × 4–7 μm vs. 26–37 × 6–9 μm for L. dictyospora and L. aquatica respectively). Lolia dictyospora has smaller ascospore than Massariosphaeria typhicola (14–19 × 4–7 μm vs. 36–51 × 9–10.5 μm for L. dictyospora and M. typhicola respectively). Barr (1990) transferred Massariosphaeria species with dictyospores to Karstenula Speg. (Didymosphaeriaceae, Melanommatales), based on their melanommataceous centrum. Karstenula species differ from L. dictyospora in having clypeate ascomata surrounded by well-developed subiculum and cylindrical asci with uniseriate ascospores (Barr 1990, Tanaka et al. 2015). Karstenula is phylogenetically distant from Lolia, where multigene phylogenetic analyses placed Karstenula species in Didymosphaeriaceae (Ariyawansa et al. 2014, Tanaka et al. 2015). Lolia species are reminiscent of Massariosphaeria in having yellow-brown, phragmospores with a large gelatinous sheath. Molecular phylogenetics analyses of ribosomal genes proved that Massariosphaeria is polyphyletic (Wang et al. 2007, Zhang et al. 2009, Hirayama et al. 2010, this study). Lolia is different from Massariosphaeria in having smaller ascospores and fewer transverse septa. Massariosphaeria species generally have ascospores with more than seven transverse septa (Tanaka & Harada 2004), while Lolia species have 3 to 6 transverse septa. Lolia species are different from Leptosphaeria species in having a prominent large gelatinous ascospore sheath. The gelatinous sheath is absent in Leptosphaeria species (Crivelli 1983, Ahn & Shearer 1998). Ariyawansa et al. (2015b) studied the phylogeny of 35 strains of Leptosphaeria representing 16 species including seven isolates of the type species L. doliolum (Pers.) Ces. & De Not. Phylogenetic analyses proved that Leptosphaeria is polyphyletic and they introduced three new genera. L. doliolum has 3-septate, brown to reddish-brown ascospores without a gelatinous sheath (Ariyawansa et al. 2015b). Lolia is phylogenetically distant from the genera: Leptosphaeria (Leptosphaeriaceae) and Massariosphaeria (Pleosporales, incertae sedis).

 

Freshwater distribution: Egypt (Abdel-Aziz 2016)

 

Fig 1. Lolia dictyospora (holotype, CBS H-22131). a Vertical section of ascomata. b Ascomatal squash showing pseudoparaphyses and mature asci. c–d Mature asci. e–h Variously shaped ascospores with prominent gelatinous sheath and glabrous gelatinous structures (arrowed). Scale bars: A = 50 μm, B = 15 μm, C–D = 10 μm, E–h = 5 μm. (Abdel-Aziz 2016; https://www.researchgate.net/publication/305276500_The_genus_Lolia_from_freshwater_habitats_in_Egypt_with_one_new_species)

 

References

Abdel-Aziz FA (2016) The genus Lolia from freshwater habitats in Egypt with one new species. Phytotaxa 267:279–288

Abdel-Aziz FA, Abdel-Wahab MA (2010) Lolia aquatica gen. et sp. nov. (Lindgomycetaceae, Pleosporales), a new coelomycete from freshwater habitats in Egypt. Mycotaxon 114:33–42

Ahn Y, Shearer CA (1998) Reexamination of taxa in Leptosphaeria originally described on host species in Ranunculaceae, Papaveraceae, and Magnoliaceae. Canadian Journal of Botany 76:258–280

Ariyawansa HA, Camporesi E, Thambugala KM, Mapook A, Kang JC, Alias SA, Chukeatirote E, Thines M, McKenzie EHC, Hyde KD (2014) Confusion surrounding Didymosphaeria-phylogenetic and morphological evidence suggest Didymosphaeriaceae is not a distinct family. Phytotaxa 176:102–119

Ariyawansa HA, Phukhamsakda C, Thambugala KM, Bulgakov TS, Wanasinghe DN, Perera RH, Mapook A, Camporesi E, Kang JC, Jones EBG, Bahkali AH, Jayasiri SC, Hyde KD, Liu ZY, Bhat JD (2015b) Revision and phylogeny of Leptosphaeriaceae. Fungal Diversity 74:19–51

Barr ME (1990) Melanommatales (Loculoascomycetes). North American Flora Series II 13:1–129

Crivelli PG (1983) Ueber die heterogen Ascomycetengattung Pleospora RABH.; Vorschlag für eine Aufteilung. Dissert ETH Zurich 7318:1–213

Dong W, Wang B, Hyde KD, McKenzie EHC, Raja HA, Tanaka K, Abdel-Wahab MA, Abdel-Aziz FA, Doilom M, Phookamsak R, Hongsanan S, Wanasinghe DN, Yu X-D, Wang G-N, Yang H, Yang J, Thambugala KM, Tian Q, Luo Z-L, Yang J-B, Miller AN, Fournier J, Boonmee S, Hu DM, Nalumpang S, Zhang H (2020) Freshwater Dothideomycetes. Fungal Diversity 105:319-575

Hirayama K, Tanaka K, Raja HA, Miller AN, Shearer CA (2010) A molecular phylogenetic assessment of Massarina ingoldiana sensu lato. Mycologia 102:729–746

Tanaka K, Harada Y (2004) Pleosporales in Japan (4). The genus Massariosphaeria. Mycoscience 45:96–105

Tanaka K, Hirayama K, Yonezawa H, Sato G, Toriyabe A, Kudo H, Hashimoto A, Matsumura M, Harada Y, Kurihara Y (2015) Revision of the Massarineae (Pleosporales, Dothideomycetes). Stud Mycol 82:75–136

Wang HK, Aptroot A, Crous PW, Hyde KD, Jeewon R (2007) The polyphyletic nature of Pleosporales: an example from Massariosphaeria based on rDNA and RBP2 gene phylogenies. Mycological Research 111:1268-1276

Zhang Y, Schoch CL, Fournier J, Crous PW, De Gruyter J, Woudenberg JHC, Hirayama K, Tanaka K, Pointing SB, Spatafora JW, Hyde KD (2009) Multi-locus phylogeny of Pleosporales: a taxonomic, ecological and evolutionary re-evaluation. Stud Mycol 64:85–102

 

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