Longipedicellata aquatica W. Dong, H. Zhang & K.D. Hyde
Index Fungorum number: IF557915; Facesoffungi number: FoF09257
Etymology: referring to aquatic habitat of this fungus
Holotype: MFLU 17-1686
Saprobic on submerged wood in freshwater. Sexual morph: Ascomata 150–200 μm high, 200–250 μm diam., lenticular or subglobose, immersed or semi-immersed, covered with a blackened pseudoclypeus, black, with mass of grey-white ascospores pouring out when mature, scattered or clustered, coriaceous, ostiolate. Peridium 5–10 μm thick, comprising several layers of brown to black, thin-walled cells of textura angularis. Pseudoparaphyses sparse, cellular, hyphae-like, hyaline, sparsely septate. Asci 85–120 × 22–27 μm (x̅ = 100 × 24 μm, n = 20), 8-spored, bitunicate, fissitunicate, narrowly to broadly clavate, elongate soon when released in water, up to 180 μm long, with a long, straight or twisted pedicel and an ocular chamber. Ascospores 19–22 × 8.5–10.5 μm (x̅ = 2 × 9.5 µm, n = 20), bi- to tri-seriate, ellipsoidal to broadly fusiform, rounded at both ends, obtuse, hyaline, 1-septate, constricted at the septum, the upper cell slightly broader and shorter than the lower cell, guttulate, with two prominent guttulate in each cell, becoming one big guttulate when mature, thin-walled, surrounded by a large, obvious, ellipsoidal, mucilaginous sheath, 7–27 μm wide, deeply constricted near the ascospores septa. Asexual morph: Undetermined. (Descriptions from Dong et al. 2020)
Culture characteristics: On PDA, colony filamentous, reaching 20 mm in 15 days at 25°C, black with brown margin from above, black from below, surface fluffy, with aerial mycelium, raised, with a smooth edge.
Material examined: THAILAND, Chiang Mai Province, Phrao District, on submerged wood in a stream, 1 September 2017, G.N. Wang, g7 (MFLU 17-1686, holotype), ex-type living culture MFLUCC 17-2334; Nakhon Phaanon Province, on submerged decaying wood of unidentified plants, 12 December 2018, H. Yang, T12 (MFLU 19-0530), living culture MFLUCC 19-0324; Mukdahan Province, on submerged decaying wood of unidentified plants, 13 December 2018, H. Yang, T45 (MFLU 19-0551), living culture MFLUCC 19-0340.
Notes: Longipedicellata aquatica clusters with L. aptrootii with high bootstrap support in the phylogenetic analysis of Dong et al. (2020). The asci of L. aquatica quickly become elongate when released in water, up to 180 μm long, but not in L. aptrootii (Zhang et al. 2016). Additionally, their ascospore sheaths are different (large, obvious, ellipsoidal, mucilaginous sheath, deeply constricted near the ascospore septa in L. aquatica vs. large, irregular, mucilaginous sheath in L. aptrootii). Longipedicellata aquatica is more similar to one collection of L. aptrootii (MFLU 16–0032) in having similar ascal size but differs in having shorter ascospores (19–22 × 8.5–10.5 μm vs. 19–26 μm × 7–11 μm) (Phukhamsakda et al. 2016). After comparision of their single genes, there are three and 21 nucleotide differences in LSU and ITS sequence data, between L. aquatica MFLUCC 17-2334 and L. aptrootii MFLUCC 10-0297, respectively, which indicates that they are distinct species (Jeewon and Hyde 2016). (Notes from Dong et al. 2020)