Pleosporales » Lophiostomataceae » Neotrematosphaeria

Neotrematosphaeria biappendiculata

Neotrematosphaeria biappendiculata (Kaz. Tanaka, Y. Harada & M.E. Barr) Thambug., Kaz. Tanaka & K.D. Hyde

Index Fungorum number: IF 551243; Facesoffungi number: FoF00810

Basionym: Trematosphaeria biappendiculata Kaz. Tanaka, Y. Harada & M.E. Barr, Fungal Diversity 19: 149 (2005)

Holotype: HHUF 28026

Etymology: From the Latin prefix bi- meaning “two” and appendiculatus meaning “appendaged” in reference to the appendages at both ends of ascospores.


Saprobic on dead twigs in freshwater habitats. Sexual morph: Ascomata 450–860μm high, 750–980μm diam., solitary, semi-immersed or slightly erumpent, coriaceous to carbonaceous, black, subglobose, ostiolate. Ostiole rounded, central, broadly papillate, with a crest-like apex and a pore-like opening. Peridium 55–100μm wide at sides, poorly developed at the base, composed of a single stratum, with 15–25 layers of dark brown to black, cells of textura prismatica, cells towards the inside lighter and at the outside, darker, fusing and indistinguishable from the host tissues. Hamathecium comprising 1–1.5μm wide, filiform, cellular, pseudoparaphyses, branching above and between asci, embedded in a gelatinous matrix. Asci 112.5–152.5×15.5–20μm ( = 133.6 × 17.6μm, n = 27), 8-spored, bitunicate, fissitunicate, cylindric-clavate, with a long pedicel, rounded at the apex, with an ocular chamber. Ascospores (28–)31–37(–40.5) × (8–)9–10.5(–12) μm ( = 33.6 × 9.8μm, n = 172), L/W (3–)3.2–3.7(–3.9) ( = 3.4, n = 172), uni to bi-seriate, partially overlapping, pale yellowish to olivaceous brown, except at the pale end cells, fusiform, mostly curved, 5(–7)-septate, slightly constricted at the septa, cell above central septum widest, with a primary submedian septum (0.50–0.54; = 0.52, n=161), smooth-walled, with appendages at both ends. Appendages globose to conical, 0.5–3μm diam., staining blue-black in Indian ink. Asexual morph: “Pleurophomopsis”-like. (Descriptions from Thambugala et al. 2015)


Material examined: JAPAN, Aomori, Hirosaki, Aoki, Mohei Pond, on submerged wood, 3 May 2003, K. Tanaka & N. Asama, KT 1124 (HHUF 28026, holotype); extype living culture, MAFF 239297, JCM 12849; Aomori, Hirosaki, Matsuzaki, Serisawa Park (pond), on submerged wood, 7 December 2002, K. Tanaka & N. Asama, KT 975 (HHUF 28017, paratype); ex-paratype living culture MAFF 239296, JCM 12850.