Pleosporales » Nigrogranaceae » Nigrograna

Nigrograna cangshanensis

Nigrograna cangshanensis Z.L. Luo, H.Y. Su & K.D.Hyde

Index Fungorum number: IF 552681; Facesoffungi number: FoF 2888

Etymology: With reference to the collection site of this fungus.

Holotype: HKAS 83978

 

Saprobic on decaying wood. Sexual morph: Ascomata 120–135 × 135–155 μm, perithecioid, gregarious, immersed, subglobose or ellipsoidal, black, with an ostiole, neck long. Ostiole mostly central, brittle. Peridium 3-layered, composed of angular cells, outer layer, dark brown, thick-walled cells, middle layer and inner layer, pale brown, with thick-walled cells. Hamathecium composed of 1.5–2.5 μm (x̄ = 2 μm, n = 25) wide, numerous, tapering distally, pseudoparaphyses, longer than asci, embedded in a gelatinous matrix. Asci 50–70 μm long (x̄ = 60 μm, n = 25), 6.5–8.5 μm wide (x̄ = 7.5 μm, n = 25), 8-spored, bitunicate, fissitunicate, clavate to cylindric-clavate, short pedicellate, apically rounded, sometimes with an ocular chamber. Ascospores 11–14 μm long (x̄ = 12.5 μm, n = 35), 3.5–4.5 μm wide (x̄ = 4 μm, n = 35), biseriate, olive-gray to yellowish-brown, 3-septate, constricted at the septa. Ascospores germinate from germ tubes from all four cells. Asexual morph: Undetermined. (Descriptions from Tibpromma et al. 2017)

 

Material examined: CHINA, Yunnan Province, saprobic on decaying wood submerged in stream in Cangshan Mountain, March 2014, Z.L. Luo, LQXM 25 (HKAS83978, holotype), ex-type living culture, MFLUCC 15-0253.

 

Notes: All species of Nigrograna are morphologically very similar and thus, Jaklitsch and Voglmayr (2016) considered them to be cryptic species. Morphologically, N. cangshanensis is very similar to other species in the genus. However, N. cangshanensis differs from N. fuscidula, N. norvegica and N. obliqua in having a thicker peridium. Phylogenetic analysis also showed that N. cangshanensis is distinct from other Nigrograna species. (Notes from Tibpromma et al. 2017)

 

Freshwater distribution: China (Luo et al. 2016)

 

Fig 1. Nigrograna cangshanensis (HKAS 83978, holotype). a Wood. b, c Appearance of ascomata on wood. d Section of ascoma. e Section of the peridium. f Paraphyses. g–k Asci. l–p Ascospores. q–s Germinating ascospore. t, u Culture grow on MEA. Scale bars d = 100 µm, e = 50 µm, f = 20 µm, g–k = 10 µm, l–p = 5 µm, q–s = 10 µm (Dong et al. 2020)

 

References

Dong W, Wang B, Hyde KD, McKenzie EHC, Raja HA, Tanaka K, Abdel-Wahab MA, Abdel-Aziz FA, Doilom M, Phookamsak R, Hongsanan S, Wanasinghe DN, Yu XD, Wang GN, Yang H, Yang J, Thambugala KM, Tian Q, Luo ZL, Yang JB, Miller AN, Fournier J, Boonmee S, Hu DM, Nalumpang S, Zhang H (2020) Freshwater Dothideomycetes. Fungal Divers 105:319–575. https://doi.org/10.1007/s13225-020-00463-5

Jaklitsch WM, Voglmayr H (2016) Hidden diversity in Thyridaria and a new circumscription of the Thyridariaceae. Stud Mycol 85:35–64. https://doi.org/10.1016/j.simyco.2016.09.002

Tibpromma S, Hyde KD, Jeewon R, Maharachchikumbura SSN, Liu JK, Bhat DJ, Jones EBG, McKenzie EHC, Camporesi E, Bulgakov TS, Doilom M, de Azevedo Santiago ALCM, Das K, Manimohan P, Gibertoni TB, Lim YW, Ekanayaka AH, Thongbai B, Lee HB, Yang JB, Kirk PM, Sysouphanthong P, Singh SK, Boonmee S, Dong W, Raj KNA, Latha KPD, Phookamsak R, Phukhamsakda C, Konta S, Jayasiri SC, Norphanphoun C, Tennakoon DS, Li J, Dayarathne MC, Perera RH, Xiao Y, Wanasinghe DN, Senanayake IC, Goonasekara ID, de Silva NI, Mapook A, Jayawardena RS, Dissanayake AJ, Manawasinghe IS, Chethana KWT, Luo ZL, Hapuarachchi KK, Baghela A, Soares AM, Vizzini A, Meiras-Ottoni A, Mešić A, Dutta AK, de Souza CAF, Richter C, Lin CG, Chakrabarty D, Daranagama DA, Lima DX, Chakraborty D, Ercole E, Wu F, Simonini G, Vasquez G, da Silva GA, Plautz HL, Ariyawansa HA, Lee H, Kušan I, Song J, Sun J, Karmakar J, Hu K, Semwal KC, Thambugala KM, Voigt K, Acharya K, Rajeshkumar KC, Ryvarden L, Jadan M, Hosen MI, Mikšík M, Samarakoon MC, Wijayawardene NN, Kim NK, Matočec N, Singh PN, Tian Q, Bhatt RP, de Oliveira RJV, Tulloss RE, Aamir S, Kaewchai S, Marathe SD, Khan S, Hongsanan S, Adhikari S, Mehmood T, Bandyopadhyay TK, Svetasheva TY, Nguyen TTT, Antonín V, Li WJ, Wang Y, Indoliya Y, Tkalčec Z, Elgorban AM, Bahkali AH, Tang AMC, Su HY, Zhang H, Promputtha I, Luangsa-ard J, Xu J, Yan J, Ji-Chuan K, Stadler M, Mortimer PE, Chomnunti P, Zhao Q, Phillips AJL, Nontachaiyapoom S, Wen TC, Karunarathna SC (2017) Fungal diversity notes 491–602: taxonomic and phylogenetic contributions to fungal taxa. Fungal Divers 83:1–261. https://doi.org/10.1007/s13225-017-0378-0

 

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