Pseudoastrosphaeriella bambusae
Pseudoastrosphaeriella bambusae Phook. & K.D. Hyde
Index Fungorum number: IF551644; Facesoffungi number: FoF 01236
Holotype: MFLU 11-0155
Etymology: The epithet “bambusae” refers to the host, of which the species was collected
Saprobic on submerged bamboo, visible as numerous, raised, black, knob-like structures with short papilla on the host surface. Sexual morph: Ascostromata 180–330 μm high, 400–890 μm diam., dark brown to black, scattered, rarely clustered, solitary to gregarious, immersed in host cortex, becoming raised, erumpent through host tissue by papilla, hemisphaerical to lenticular, or depressed conical, with flattened base, uni-loculate, rarely bi-loculate, glabrous, coriaceous, ostiolate, with short papilla. Papilla 170–250 μm high, 120–180 μm wide, black, central, cylindrical, straight, internally periphysate, brittle, carbonaceous, arranged vertically in a textura prismatica. Peridium 15–65 μm wide, of unequal thickness, thick at the sides, especially towards the apex, composed of dark brown to black, pseudoparenchymatous cells, comprising host cells plus fungal tissue at outside, internally arranged in a textura angularis, poorly developed at the base (15–40 μm wide), with palisade-like cells at the rim. Hamathecium composed of dense, 1–1.8 μm wide, filiform, trabeculate pseudoparaphyses, anastomosing at the apex, embedded in a hyaline gelatinous matrix. Asci (102–)105–130(–136) × (14.5–)15–18(–19.5) μm (x̄ = 119.7 × 16.1 μm, n = 30), 8-spored, bitunicate, cylindricclavate to clavate, with furcate or foot-like pedicel, apically rounded, with well-developed ocular chamber. Ascospores (37–)(39–)41–44(–48) × 5–7 μm (x̄ = 42.5 × 6.5 μm, n = 30), overlapping uni-seriate at the base, bito tri-seriate at the apex, slightly reddish brown, fusiform, with acute or rounded ends, 1(–3)-septate, slightly constricted at the central septum, swollen above central septum, rough-walled with minute striations, occasionally upper cell shorter than lower cell, surrounded by mucilaginous sheath. Asexual morph: Undetermined. (Description from Phookamsak et al. 2015)
Material examined: THAILAND, Chiang Rai Province, Mae Jun District, Huai Kang Pla Waterfall, on dead branches of submerged bamboo, 25 October 2010, R. Phookamsak, RP0034 (MFLU 11-0155, holotype), extype living culture, MFLUCC 11-0205
Notes: Pseudoastrosphaeriella bambusae is most similar to P. africana and P. thailandensis. However, P. bambusae differs from P. africana and P. thailandensis in having reddish brown ascospores, with inconspicuous striations, while P. africana and P. thailandicum having slightly dark brown ascospores, with wide, distinct longitudinal ridges. Pseudoastrosphaeriella bambusae forms a robust clade (75 % MP, 91%ML, 1.00 PP), closely related to P. thailandensis in the phylogenetic analyses. (Notes from Phookamsak et al. 2015)
Freshwater distribution: Thailand (Phookamsak et al. 2015, Dong et al. 2020)
Fig. 1. Pseudoastrosphaeriella bambusae (MFLU 11-0155, holotype). a, b Appearance of ascostromata on host surface. c Section through ascostroma. d, e Section through peridium. f Papilla with internal periphysate. g Trabeculate pseudoparaphyses. h Asci embedded in trabeculate pseudoparaphyses. i–k Asci. l–o Ascospores. p Ascospore stained in Indian ink. q, r Culture characters (q = from above, r = from below). Scale bars: c = 200 μm, d–k = 20 μm, l–p = 10 μm. (Phookamsak et al. 2015)
References
Phookamsak R, Norphanphoun C, Tanaka K, Dai DQ, Luo ZL, Liu JK, Su HY, Bhat DJ, Bahkali AH, Mortimer PE (2015) Towards a natural classification of Astrosphaeriella-like species; introducing Astrosphaeriellaceae and Pseudoastrosphaeriellaceae fam. nov. and Astrosphaeriellopsis, gen. nov. Fungal Divers 74:143–197
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