Pleosporales » Roussoellaceae » Neoroussoella

Neoroussoella leucaenae

Neoroussoella leucaenae Jayasiri, E.B.G. Jones & K.D. Hyde

Index Fungorum number: IF 555570; Facesoffungi number: FoF 05277

Holotype: MFLU 18–2159

Etymology: Referring to the host genus on which the fungus was collected, Leucaena (Fabaceae)

 

Saprobic on submerged wood. Sexual morph: Ascostromata 140–180 μm high, 110–170 diam., immersed to erumpent, scattered, raised, visible as black, dome-shaped spots and with prominent, elongate neck, coriaceous, black, ostiolate. Ostiole 60–90 μm diam., rounded, brown to dark brown, central. Peridium 10–15 μm wide, composed of several layers of compressed cells of textura angularis, dark brown to black in outer layer, hyaline inwardly, flattened at the base. Pseudoparaphyses 3 μm wide, numerous, cellular, narrow, filamentous, indistinctly septate, anastomosing, embedded in a gelatinous matrix. Asci 65–145 × 4.7–6.5 μm (x̄ = 98 × 5.5 μm, n = 10), 8-spored, bitunicate, cylindrical, pedicellate, apically rounded with an ocular chamber, elongate when in water. Ascospores 7.5–10.7 × 3–4.5 μm (x̄ = 8.8 × 3.5 μm, n = 30), overlapping uniseriate, ellipsoidal to broadly fusiform, 1-septate, constricted at the septum, pale brown to brown, smooth-walled. Asexual morph: Undetermined. (Description from Dong et al. 2020)

 

Material examined: CHINA, Yunnan Province, Pingbian, on submerged wood in a stream, 20 September 2017, W. Dong, WF11A-1 (HKAS 101701), living culture KUMCC 18-0050; ibid., WF11A-2 (MFLU 18-1175).

 

Notes: Our collection KUMCC 18-0050 clusters with Neoroussoella leucaenae with high bootstrap support. KUMCC 18-0050 is identified as N. leucaenae based on sequence data which has 100% similarity in ITS sequence data, and one nucleotide difference in TEF sequence data, with the ex-type strain MFLUCC 18-1544. Our collection has longer asci (65–145 × 4.7–6.5 μm vs. 50–60 × 4.5–5.5 μm) than the holotype (Jayasiri et al. 2019). This is a new habitat and geographical record for N. leucaenae from freshwater in China. (Notes from Dong et al. 2020)

 

Freshwater distribution: China (Dong et al. 2020)

 

Fig. 1 Neoroussoella leucaenae (HKAS 101701, new habitat and geographical record). a, b Ascostromata on host substrate. c, d Vertical section of ascomata. e Structure of peridium. f Pseudoparaphyses. g–j Bitunicate asci. k–m Ascospores. n Colony on PDA (from front). Scale bars: c, d = 50 μm, e, g–j = 20 μm, f = 10 μm, k–m = 5 μm. (Dong et al. 2020)

 

References

Dong W, Wang B, Hyde KD, McKenzie EHC, Raja HA, Tanaka K, Abdel-Wahab MA, Abdel-Aziz FA, Doilom M, Phookamsak R, Hongsanan S, Wanasinghe DN, Yu X-D, Wang G-N, Yang H, Yang J, Thambugala KM, Tian Q, Luo Z-L, Yang J-B, Miller AN, Fournier J, Boonmee S, Hu D-M, Nalumpang S, Zhang H (2020) Freshwater Dothideomycetes. Fungal Divers 105:319–575

Jayasiri SC, Hyde KD, Jones EBG, McKenzie EHC, Jeewon R, Phillips AJL, Bhat DJ, Wanasinghe DN, Liu JK, Lu YZ, Kang JC, Xu JC, Karunarathna SC (2019) Diversity, morphology and molecular phylogeny of Dothideomycetes on decaying wild seed pods and fruits. Mycosphere 10:1–186

 

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