Pleosporales » Tetraplosphaeriaceae » Tetraploa

Tetraploa puzheheiensis

Tetraploa puzheheiensis W. Dong, H. Yang & H. Zhang

Index Fungorum number: IF557936

Holotype: MFLU 20-0441

 

Saprobic on submerged wood. Sexual morph: Undetermined. Asexual morph: Mycelium superficial. Conidiophores absent. Conidiogenous cells monoblastic. Conidia 24–35 × 15–24.5 μm (x̄ = 28.5 × 17.5 μm, n = 40), solitary, short cylindrical, dark brown to black, verrucose, consisting of 2–3 columns of 7–9.5 µm wide, 4–5-celled, with 1–3 appendages. Appendages 3–27 µm long, 3–4.5 µm wide at the base, 2–3.5 µm wide at the apex, 0–2-septate, dark brown at the base and hyaline to pale brown at the apex, smooth, unbranched, straight. (Descriptions from Dong et al. 2020)

 

Cultural characteristics: Conidia germinated on PDA within 24 hours, germtubes produced from apex of conidia. Colonies on PDA reaching 18–20 mm diam. in 18 days at 25 °C, with dense, floccose, brown mycelium on the surface, reverse brown to dark brown, with undulate, pale brown margin.

 

Material examined: CHINA, Yunnan Province, Puzhehei Wetland, 24°8ʹ32ʺN, 104°6ʹ58ʺE, on submerged wood, 12 May 2018, H. Yang, P12 (MFLU 20-0441, holotype), ex-type living culture KUMCC 20-0151.

 

Notes: The collection of Dong et al. (2020), KUMCC 20-0151, clusters with two putative strains of Tetraploa aristata (CBS 996.70) and T. scheueri (CY112) with high bootstrap support. Tetraploa scheueri CY112 was sequenced by Rodrigues et al. (2011), without morphological description. However, KUMCC 20-0151 differs from the holotype of T. scheueri GZU 36-91 in having shorter appendages (3–27 µm vs. 263–350 µm) with less septa (0–2-septate vs. 17–22-septate) (Tanaka et al. 2009). The morphology of T. aristata CBS 996.70 also cannot be compared as the conidia were not produced in the culture (Tanaka et al. 2009). Ellis (1949) reported two types of conidia of T. aristata, however, KUMCC 20-0151 only has one type of conidia. Although type A of T. aristata has similar conidial size with KUMCC 20-0151, T. aristata differs in having longer appendages (12–80 µm vs. 3–27 µm) with more septa (1–6-septate vs. 0–2-septate). In addition, there are only two nucleotide differences in ITS sequence data between T. aristata (CBS 996.70) and T. scheueri (CY112), which indicates that these two strains are very likely to be the same species and at least one strain was misidentified. The real identities of CBS 996.70 and CY112 cannot be resolved until their epitypification with sufficient DNA sequence data are obtained in future. Given this, we introduce KUMCC 20-0151 as a new species T. puzheheiensis. Two strains CBS 996.70 and CY112 are named as Tetraploa spp. in the phylogenetic tree of Dong et al. (2020). (Notes from Dong et al. 2020)

 

Freshwater distribution: China (Dong et al. 2020)

 

Fig 1. Tetraploa puzheheiensis (MFLU 20-0441, holotype). a, b Colonies on natural substrate. c–j Conidia. k Germinated conidium. l, m Colony on PDA (up-front, down-reverse). Scale bars: c–k = 10 μm (Dong et al. 2020)

 

References

Dong W, Wang B, Hyde KD, McKenzie EHC, Raja HA, Tanaka K, Abdel-Wahab MA, Abdel-Aziz FA, Doilom M, Phookamsak R, Hongsanan S, Wanasinghe DN, Yu X-D, Wang G-N, Yang H, Yang J, Thambugala KM, Tian Q, Luo Z-L, Yang J-B, Miller AN, Fournier J, Boonmee S, Hu D-M, Nalumpang S, Zhang H (2020) Freshwater Dothideomycetes. Fungal Divers 105:319–575. https://doi.org/10.1007/s13225-020-00463-5

Ellis MB (1949) Tetraploa. Trans Br Mycol Soc 32:246–251. https://doi.org/10.1016/s0007-1536(49)80013-1

Rodrigues A, Mueller UG, Ishak HD, Bacci M, Pagnocca FC (2011) Ecology of microfungal communities in gardens of fungus-growing ants (Hymenoptera: Formicidae): A year-long survey of three species of attine ants in Central Texas. FEMS Microbiol Ecol 78:244–255. https://doi.org/10.1111/j.1574-6941.2011.01152.

Tanaka K, Hirayama K, Yonezawa H, Hatakeyama S, Harada Y, Sano T, Shirouzu T, Hosoya T (2009) Molecular taxonomy of bambusicolous fungi: Tetraplosphaeriaceae, a new pleosporalean family with Tetraploa-like anamorphs. Stud Mycol 64:175–209. https://doi.org/10.3114/sim.2009.64.10

 

 

Recent Genus

1
Neochlamydotubeufia
Neohelicomyces

Recent Species

Neohelicomyces submersus
Neohelicomyces hyalosporus
Neohelicomyces grandisporus