Pleosporales » Tetraplosphaeriaceae » Tetraploa

Tetraploa yunnanensis

Tetraploa yunnanensis W. Dong, H. Yang & H. Zhang

Index Fungorum number: IF557937; Facesoffungi number: FoF09282

Etymology: referring to Yunnan province, where the holotype was collected

Holotype: MFLU 19-0524

 

Saprobic on submerged wood. Sexual morph: Undetermined. Asexual morph: Mycelium superficial. Conidiophores absent. Conidiogenous cells monoblastic. Conidia 30–50 × 16–22 μm (x̄ = 43 × 19 μm, n = 10), solitary, short to long cylindrical, brown to dark brown, verrucose, consisting of 2–3 columns of 7.5–10 µm wide, 4–6-celled, with 1–3 appendages. Appendages 25–83 µm long, 4–5.5 µm wide at the base, 1.5–2.5 µm wide at the apex, 1–5-septate, brown, smooth, unbranched, straight. (Descriptions from Dong et al. 2020)

 

Cultural characteristics: Conidia germinated on PDA within 24 hours. Colonies on PDA reaching 20–25 mm diam. in 18 days at 25 °C, with dense, floccose, brown mycelium on the surface, reverse brown to dark brown, with undulate, pale brown margin.

 

Material examined: CHINA, Yunnan Province, Puzhehei Wetland, 24°8ʹ32ʺN, 104°6ʹ58ʺE, on submerged wood, 12 May 2018, H. Yang, P13 (MFLU 19-0524, holotype), ex-type living culture MFLUCC 19-0319; Thailand, Chiang Mai Province, on submerged wood, 9 February 2018, G.N. Wang, A66 (MFLU 18-0959), living culture MFLUCC 18-0652.

 

Notes: Tetraploa yunnanensis clusters with a strain of T. sasicola (NCYUCC 19-0371) with high bootstrap support (Dong et al. 2020). Tetraploa sasicola NCYUCC 19-0371 (MFLU 19-2698) is an ascomycetous species characterized by hyaline to yellowish brown ascospores without appendages (Hyde et al. 2020). Although asexual morph of NCYUCC 19-0371 was not formed in culture (Hyde et al. 2020), phylogeny clearly separates them as distinct species (Dong et al. 2020). Dong et al. (2020) found that NCYUCC 19-0371 clusters in a separated clade between Tetraploa yunnanensis (MFLUCC 19-0319, ex-type strain) and T. sasicola (KT 563, ex-type strain) with high bootstrap support. Comparison of single LSU and ITS sequence data also shows 14 and 25 nucleotide differences between NCYUCC 19-0371 and KT 563, respectively. This indicates that NCYUCC 19-0371 and KT 563 are phylogenetically different species (Jeewon and Hyde 2016).

 

NCYUCC 19-0371 differs from KT 563 in having larger asci (97–109 × 22–23 μm vs. 61–100 × 9–13 μm) and wider ascospores (24–29 × 4–14 μm vs. 22.5–34 × 3–5 μm). Additionally, NCYUCC 19-0371 has hyaline to yellowish brown ascospores without appendages, while KT 563 has hyaline ascospores with a narrow sheath drawn out at both ends, which is longer at the apex than the one at the base (Tanaka et al. 2009; Hyde et al. 2020). NCYUCC 19-0371 bearing the name Tetraploa sasicola is probably a novel species, but the introduction should be performed with re-examination of the specimen MFLU 19-2698 or new collections. (Notes from Dong et al. 2020)

 

Fig 1. Tetraploa yunnanensis (MFLU 19-0524, holotype). a, b Colonies on natural substrate, c–h Conidia, i Germinated conidium, j, k Colony on PDA (up-front, down-reverse). Scale bars: c–i = 20 μm (Dong et al. 2020)

 

References

Dong W, Wang B, Hyde KD, McKenzie EHC, Raja HA, Tanaka K, Abdel-Wahab MA, Abdel-Aziz FA, Doilom M, Phookamsak R, Hongsanan S, Wanasinghe DN, Yu X-D, Wang G-N, Yang H, Yang J, Thambugala KM, Tian Q, Luo Z-L, Yang J-B, Miller AN, Fournier J, Boonmee S, Hu D-M, Nalumpang S, Zhang H (2020) Freshwater Dothideomycetes. Fungal Divers 105:319–575. https://doi.org/10.1007/s13225-020-00463-5

Hyde KD, Dong Y, Phookamsak R, Jeewon R, Bhat DJ, Jones EBG, Liu N-G, Abeywickrama PD, Mapook A, Wei D, Perera RH, Manawasinghe IS, Pem D, Bundhun D, Karunarathna A, Ekanayaka AH, Bao D-F, Li J, Samarakoon MC, Chaiwan N, Lin C-G, Phutthacharoen K, Zhang S-N, Senanayake IC, Goonasekara ID, Thambugala KM, Phukhamsakda C, Tennakoon DS, Jiang H-B, Yang J, Zeng M, Huanraluek N, Liu J-K (Jack), Wijesinghe SN, Tian Q, Tibpromma S, Brahmanage RS, Boonmee S, Huang S-K, Thiyagaraja V, Lu Y-Z, Jayawardena RS, Dong W, Yang E-F, Singh SK, Singh SM, Rana S, Lad SS, Anand G, Devadatha B, Niranjan M, Sarma VV, Liimatainen K, Aguirre-Hudson B, Niskanen T, Overall A, Alvarenga RLM, Gibertoni TB, Pfliegler WP, Horváth E, Imre A, Alves AL, da Silva Santos AC, Tiago PV, Bulgakov TS, Wanasinghe DN, Bahkali AH, Doilom M, Elgorban AM, Maharachchikumbura SSN, Rajeshkumar KC, Haelewaters D, Mortimer PE, Zhao Q, Lumyong S, Xu J, Sheng J (2020) Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Divers 100:5–277. https://doi.org/10.1007/s13225-020-00439-5

Jeewon R, Hyde KD (2016) Establishing species boundaries and new taxa among fungi: Recommendations to resolve taxonomic ambiguities. Mycosphere 7:1669–1677. https://doi.org/10.5943/mycosphere/7/11/4

Tanaka K, Hirayama K, Yonezawa H, Hatakeyama S, Harada Y, Sano T, Shirouzu T, Hosoya T (2009) Molecular taxonomy of bambusicolous fungi: Tetraplosphaeriaceae, a new pleosporalean family with Tetraploa-like anamorphs. Stud Mycol 64:175–209. https://doi.org/10.3114/sim.2009.64.10

 

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