Wicklowia submersa
Wicklowia submersa Boonmee, Sorvongxay & K.D. Hyde
Index Fungorum number: IF 556334; Facesoffungi number: FoF 05994
Etymology: The specific epithet “submersa” refers to the submerged habitats of this fungus.
Holotype: MFLU 18–1092
Saprobic on decaying submerged wood in a freshwater stream. Sexual morph: Ascomata 128–143 × 175–215 μm (x̄ = 135 × 192 μm), immersed, erumpent when mature, raising the host tissue, visible as dark oval or slit-like on host surface, uni-loculate, subglobose, dark brown, scattered, gregarious, ostiolate. Peridium 20–24 μm wide, thick-walled, composed of dark brown of pseudoparenchymatous cells, of textura angularis. Hamathecium comprising 1–2 μm wide, numerous, cylindrical, filiform, branched, septate, hyaline pseudoparaphyses. Asci 66–84 × 19–23 μm (x̄ = 77 × 21 μm, n = 10), 8-spored, bitunicate, fissitunicate, cylindrical-clavate, apically thickened and rounded, with an ocular chamber when immature, sessile or short pedicellate. Ascospores 24–27 × 7–9 μm (x̄ = 25 × 8 μm, n = 20), 2-seriate, partially overlapping, ellipsoidal-oblong with rounded ends, slightly inequilateral, asymmetrical, upper cell wider, slightly curved, hyaline, 1-septate, constricted at the septum, guttulate when immature, surrounded by a distinctly mucilaginous sheath, without any appendages, smooth-walled. Asexual morph: Undetermined. (Descriptions from Boonmee et al. 2019)
Material examined: THAILAND, Krabi Province, on decaying submerged wood of an unidentified host, in freshwater stream, 31 August 2017, Monika C. Dayarathne, MNK03 (MFLU 18–1092, holotype), ex-type culture: MFLUCC 18–0373
Notes: Wicklowia submersa shares common characteristics with the type species W. aquatica Raja, A. Ferrer & Shearer such as immersed, visible as dark oval or slit-like on host surface, uniloculate ascomata, bitunicate, fissitunicate, cylindrical-clavate asci and surrounded by mucilaginous sheath, regularly 1-septate, hyaline ascospores. However, W. submersa differs from the type species W. aquatica (Raja et al. 2010), in having short pedicellate asci, and ascospores lacking filamentous appendages at both ends. In addition, the sizes of ascomata, asci and ascospores of W. submersa are smaller than W. aquatica. Phylogenetic analysis based on combined LSU and SSU sequence data indicated that W. submersa represents a basal taxon to W. aquatica with high-support and is a distinct species in the genus. (Notes from Boonmee et al. 2019)
Freshwater distribution: Thailand (Boonmee et al. 2019)
Fig 1. Wicklowia submersa ((MFLU 18–1092, holotype). a–b Material and appearance of ascomata on host surface. c, d Vertical sections of ascomata. e Peridium. f Pseudoparaphyses. g Immature asci with an ocular chamber (arrows). h–j Mature asci. k–m Ascospores mounted in water with surrounded by a distinctly mucilaginous sheath. n Ascospores stained in India Ink reagent. o Ascospores stained in nigrosin reagent. Scale bars: a = material, b = 200 μm, c, d = 100 μm, e, g–j=20 μm, f, k–n = 10 μm. (Boonmee et al. 2019)
References
Raja HA, Ferrer A, Shearer CA, Miller AN (2010a) Freshwater ascomycetes: Wicklowia aquatica, a new genus and species in the Pleosporales from Florida and Costa Rica. Mycoscience 51:208–214
Recent Genus
1Neochlamydotubeufia
Neohelicomyces
Recent Species
Neohelicomyces submersusNeohelicomyces hyalosporus
Neohelicomyces grandisporus