Tubeufiales » Tubeufiaceae » Berkleasmium

Berkleasmium thailandicum

Berkleasmium thailandicum (Tanney & A.N. Mill.) Y.Z. Lu & K.D. Hyde

Basionym: Neoacanthostigma thailandicum Tanney & A.N. Mill., IMA Fungus 8(1): 103 (2017)

Synonymy: Neoacanthostigma brunneisporum Y.Z. Lu, Boonmee & K.D. Hyde, Cryptog. Mycol. 38(2): 179 (2017)

Index Fungorum number: IF 553171; Facesoffungi number: FoF 03240

Etymology: ‘brunneisporum’ referring to brown helicosporous conidia on woody substrate

Holotype: MFLU 17-0335

 

Saprobic on decaying wood in a freshwater stream. Sexual morph: Ascomata 190–275 μm high × 175–230 μm diam., superficial, seated on a subiculum, solitary, scattered, subglobose, ellipsoidal-ovate, dark brown to black, with a central ostiolate, covered with brown to dark brown, 45–85 μm long, septate, tapering to an acute apex setae. Peridium 20–28 μm wide, composed cells of textura angularis, brown, with innermost part comprising thin layers of hyaline to pale brown cells of textura subprismatica. Hamathecium comprising numerous, filiform, septate, branched, hyaline pseudoparaphyses. Asci 110–140 × 17–23 μm (x̄ = 125 × 20 μm, n=20), 8-spored, bitunicate, cylindrical, short-pedicellate, apically rounded. Ascospores 95–115(–128) × 4.5–6.5 μm (x̄ = 108 × 5.5 μm, n=50), fasciculate, broadly filiform, cylindrical to long subfusiform, elongate, rounded at ends, slightly curved, guttulate, 12–13-septate, not constricted at septa, hyaline to pale brown, smooth-walled. Asexual morph: hyphomycetous, helicosporous. Conidiophores 10–24 × 4–6 μm, pale brown, macronematous, erect, short, cylindrical, septate, smooth-walled. Conidiogenous cells 10–19 μm long, 4–5.5 μm wide, monoblastic, holoblastic, integrated, terminal, cylindrical, truncate at apex, hyaline to pale brown, smoothwalled. Conidia 89–155 μm diam. and conidial filament 10.5–14(–15) μm wide (x̄ = 130 × 12 μm, n = 50), 630–950 μm long, coiled 2–3 times when tightly coiled, becoming loosely coiled in the water, basal cell elongated, rounded at tip, multiseptate, up to 60-septate, slightly constricted at septa, pale brown, smooth-walled. (Descriptions from Lu et al. 2017)

 

Material examined: THAILAND, Uttaradit, Laplae, Mae Phun, Ban Ton Klua, on decaying wood in a freshwater stream, 24 October 2015, Saranyaphat Boonmee, UTD15-4 (MFLU 17-0335, holotype; GZAAS 17-0002, isotype); extype living culture, MFLUCC 16-0015 = GZCC 17-0002; Ibid., 24 October 2015, Saranyaphat Boonmee, UTD18 (MFLU 17-0337, paratype); living culture, MFLUCC 16-0012; THAILAND, Krabi, Plai Praya, Khao To, Ban Bang Thao Mae, on decaying wood in a freshwater stream, 17 December 2015, Saranyaphat Boonmee, BTM12 (MFLU 17-0334, paratype); living culture, MFLUCC 16-1127.

 

Notes: Four isolations, collected from submerged decaying wood in freshwater stream of Thailand, are identified as a new Berkleasmium species, B. thailandicum. Phylogenetically, three new collections cluster together with Berkleasmium septoconstrictum (MFLUCC 15–1248) and formed a distinct clade from B. septoconstrictum (ANM 536.1). However, Hyde et al. (2016) identified the strain MFLUCC 15-1248 as B. septoconstrictum because it clustered together with B. septoconstrictum (ANM 536.1) and this might be because of lack of sequences (gene regions). With more isolates included in the analysis, we compared our new taxa with B. septoconstrictum MFLUCC 15-1248 by using single gene data. There are only one bp difference in ITS and TEF1α respectively, which showed that they could be the same species. Moreover, we also compared our new taxa and B. septoconstrictum MFLUCC 15-1248 with B. septoconstrictum ANM 536.1 by using single gene data. There are 49 bp and 20 bp differences in ITS and LSU respectively, which confirmed that they are phylogenetically distinct species from B. septoconstrictum ANM 536.1. Therefore, we identify MFLUCC 15–1248 isolate as B. thailandicum. Morphologically, B. thailandicum differs from B. septoconstrictum in having different setae (septate vs one-celled) and longer ascospores (95-115 μm vs 40-50 μm). (Notes from Lu et al. 2017)

 

Freshwater distribution: Thailand (Lu et al. 2017)

 

 

Fig 1. Berkleasmium thailandicum (MFLU 17-0334 and MFLU 17-0337, paratypes). a Colony on decaying wood. b-d Conidiogenous cell with hyphae. e-i Conidia. j Germinating conidium. k-l Colony on MEA from above and below. Scale bars: a = 500 µm, b, e-j = 50 µm, c-d = 10 µm, k-l = 20 mm. (Lu et al. 2017)

 

References

Lu YZ, Boonmee S, Liu JK, Hyde KD, Bhat DJ, Eungwanichayapant PD, Kang JC (2017) Novel Neoacanthostigma species from aquatic habitats. Cryptogam Mycol 38:169–191

 

 

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