Chlamydotubeufia cylindrica
Chlamydotubeufia cylindrica Y.Z. Lu, Boonmee & K.D. Hyde
Index Fungorum number: IF 554820; Facesoffungi number: FoF 04699
Etymology: “cylindrica” referring to the cylindrical asci of this fungus.
Holotype: MFLU 17–1177
Saprobic on submerged decaying wood in a freshwater stream. Sexual morph: Ascomata 175–225 high × 175–250 μm diameter (x̄ = 205 × 200 μm, n = 10), superficial, solitary or scattered, globose to subglobose, black, setose, coriaceous, with a central ostiole. Ostiole single, central. Setae 40–65 × 5–6 μm, covering the whole ascoma, dark brown to black, taper to an acute apex, unbranched, thick-walled. Peridium 25–30 μm thick, composed of several layers of cells of textura angularis. Hamathecium of 1–2 μm wide, branched pseudoparaphyses. Asci 90–110 × 15–18 μm (x̄ = 100 × 16 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical to clavate, saccate, apically rounded, short-pedicellate. Ascospores 30–45 × 6–7.5 μm (x̄ = 36 × 6.5 μm, n = 50), 2–3-seriate, fusiform, hyaline, slightly curved, tapering toward the ends, enlarged at the 3rd cell, guttulate, 5–6-septate, not constricted at the septa. Asexual morph: Hyphomycetous, chlamydosporous. Conidiophores lacking. Chlamydospores 50–90 μm long, 30–45 μm wide (x̄ = 67 × 35 μm, n = 20), holoblastic, sometimes intercalary, broadly oval to ellipsoid, dictyoseptate, pale brown when immature, darkened to black when matured, with terminal cells round and subhyaline. (Description from Lu et al. 2018)
Culture characteristics: Ascospores germinating on water agar and germ tubes produced from ascospores within 12 h. Colonies growing slowly on malt extract agar (MEA), circular, with flat surface, veined and without wrinkle, edge entire, reaching 31 mm in 4 weeks at 28 °C, brown to dark brown in MEA media. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown, becoming dark brown due to the development of chlamydospores.
Material examined: THAILAND, Krabi, Plai Praya, Khao To, Ban Bang Thao Mae, on submerged decaying wood in a freshwater stream, 17 December 2015, Saranyaphat Boonmee, BTM01–2 (MFLU 17–1177, holotype; HKAS 100730, isotype), ex-type living cultures, MFLUCC 16–1130, TBRC.
Notes: Morphologically, Chlamydotubeufia cylindrica differs from C. krabiensis by its shorter but larger ascomata diameter (175–225 × 175–250 μm vs. 232–261 × 174–207 μm) and differs from C. huaikangplaensis by its longer, but narrower asci (90–110 × 15–18 μm vs. 50–83 × 16–21 μm). Its asexual morphs are hyphomycetous, chlamydosporous, obtained from sexual morph’s living cultures. The multi-gene phylogenetic analysis shows that C. cylindrica formed is basal to C. krabiensis, C. chlamydosporum and the type species C. huaikangplaensis and phylogenetically distinct with good bootstrap support (Lu et al. 2017). (Notes from Lu et al. 2018)
Fig 1. The sexual morph of Chlamydotubeufia cylindrica (MFLU 17–1177, holotype). a, b Superficial ascomata on substrate. Note ascomata surrounded by black setae. c, d Ascoma. e Peridium. f Seta. g–i Asci. j–o Ascospores. Scale bars: a, b = 200 µm, c, d = 100 µm, e–o = 20 µm. (Lu et al. 2018)
Fig 2. The asexual morph of Chlamydotubeufia cylindrica (from ex-type living cultures). a Germinating ascospore. b, c Colony on MEA from above and below. d–f Mycelium and conidiophores. g–m Chlamydospores. Scale bars: a, g–m = 20 µm, d–f = 50 µm (Lu et al. 2018)
References
Lu YZ, Liu JK (Jack), Hyde KD, Jeewon R, Kang JC, Fan C, Boonmee S, Bhat DJ, Luo ZL, Lin CG, Eungwanichayapant PD (2018) A taxonomic reassessment of Tubeufiales based on multi-locus phylogeny and morphology. Fungal Divers 92:131–344. https://doi.org/10.1007/s13225-018-0411-y
Recent Genus
1Neochlamydotubeufia
Neohelicomyces
Recent Species
Neohelicomyces submersusNeohelicomyces hyalosporus
Neohelicomyces grandisporus