Chlamydotubeufia huaikangplaensis Boonmee & K.D. Hyde
Etymology: from –ensis meaning pertaining to and Huai Kang Pla, the place of collection in reference to the collecting site.
Saprobic on decaying wood in a freshwater stream. Mycelium partly immersed in woody substratum and partly superficial, consisting of septate, branched, hyaline to brown, smooth, thin-walled hyphae. Sexual morph: Ascomata 161–192 × 142–184 μm, superficial, solitary or scattered, globose to subglobose, black, with a central ostiolate, covered with darkened and 1-celled setae. Peridium 17–23 μm thick, a single substratum, composed of 3–4 layers of cells of textura angularis. Hamathecium comprising 2–2.5 μm wide, branched, hyaline pseudoparaphyses embedded in a gelatinous matrix. Asci 50–83 × 16–21 μm, 8-spored, bitunicate, fissitunicate, cylindrical to clavate or saccate, apically rounded, short pedicellate. Ascospores 32–41.5 × 5–8 μm, fusiform, slightly curved, guttulate, 5–6-septate, slightly constricted at the septa, hyaline to pale gray. (Boonmee et al. 2011). Asexual morph: Hyphomycetous. Chlamydospores 44.5–80.5 μm long, 31–49.5 μm wide (x̄ = 64 × 38 μm, n = 20), blastic, intercalary, broadly oval to ellipsoid, dictyoseptate, sharply constricted at septa, smooth, darkened to black.
Culture characteristics: Conidia germinating on water agar (WA) within 24 h and germ tubes produced at both ends. Colonies growing slowly on malt extract agar (MEA), reaching 15 mm in 3 weeks at 28 °C, brown to dark brown in MEA media. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown, becoming dark brown due to the development of chlamydospores.
Material examined: THAILAND, Chiang Rai, Muang, Ban Nang Lae Nai, on decaying wood in a freshwater stream, 28 November 2015, Saranyaphat Boonmee and Yong-Zhong Lu, TUB02–2 (MFLU 17-0331); living culture, MFLUCC 16-0023; Krabi, Plai Praya, Khao To, Ban Bang Thao Mae, on decaying wood in a freshwater stream, 17 December 2015, Saranyaphat Boonmee, BTM01–1 (MFLU 17-0333,); living culture, MFLUCC 16-0227.
GenBank numbers: MFLUCC 16-0023 ITS:KY678766; LSU:KY678758; TEF1:KY792597. MFLUCC 16-0227 ITS:KY678765; LSU:KY678757; TEF1:KY792596.
Notes: Two new collections of Chlamydotubeufia huaikangplaensis (MFLU 17-0331 and MFLU 17-0333) were found on submerged wood in Thailand, it is the first report that C. huaikangplaensis is found from aquatic habitats. Morphologically, they can be identified to Chlamydotubeufia as they formed typical chlamydospores in woody substrate and cultures. Phylogenetic analysis of Dong et al. (2020) placed the three new strains in the same clade with C. huaikangplaensis. (Notes from Dong et al. 2020)
Fig 1 Chlamydotubeufia huaikangplaensis (MFLU 17-0331 and MFLU 17-0333). a Chlamydospores arise directly from hyphal cells on natural substrata. b Chlamydospores on MEA substrata. c–d Chlamydospores with hyphae. e–k Chlamydospores. l Germinating chlamydospores. m, n Colony on MEA from above and below. Scale bars a–b = 200 µm, c = 100 µm, d–l = 50 µm, m–n = 20 mm (Dong et al. 2020)
Boonmee S, Zhang Y, Chomnunti P, Chukeatirote E, Tsui CKM, Bahkali AH, Hyde KD (2011) Revision of lignicolous Tubeufiaceae based on morphological reexamination and phylogenetic analysis. Fungal Divers 51:63–102. https://doi.org/10.1007/s13225-011-0147-4
Dong W, Wang B, Hyde KD, McKenzie EHC, Raja HA, Tanaka K, Abdel-Wahab MA, Abdel-Aziz FA, Doilom M, Phookamsak R, Hongsanan S, Wanasinghe DN, Yu X-D, Wang G-N, Yang H, Yang J, Thambugala KM, Tian Q, Luo Z-L, Yang J-B, Miller AN, Fournier J, Boonmee S, Hu D-M, Nalumpang S, Zhang H (2020) Freshwater Dothideomycetes. Fungal Divers 105:319–575. https://doi.org/10.1007/s13225-020-00463-5