Neochlamydotubeufia khunkornensis

Neochlamydotubeufia khunkornensis (Boonmee & K.D. Hyde) Y.Z. Lu

≡ Chlamydotubeufia khunkornensis Boonmee & K.D. Hyde, Fungal Diversity 51(1): 80 (2011)

Index Fungorum number: IF 554863; Facesoffungi number: FoF 04733

Saprobic on submerged wood from freshwater habitats. Ascomata 180–250 μm high × 199–249 μm diam (x̄ =210.5 × 220μm, n = 5), superficial, solitary or scattered, globose to subglobose, with a central ostiolate 20–30 μm wide, covered with dark brown setae, which taper to an acute apex. Peridium 18–28 μm thick, composed of 3–5 layers of light brown cells of compressed textura angularis. Hamathecium numerous, cellular, cylindrical, filiform pseudoparaphyses, hyaline, embedded in a gelatinous matrix. Asci 79.5–106 × 13–21 μm (x̄ =96.5×15.5μm, n = 20), 8-spored, bitunicate, cylindrical to clavate, apically rounded, ocular chamber not observed, short pedicellate. Ascospores 34–42 × 5–7 μm (x̄ =38×6μm, n = 20), 2–3-seriate, fusiform, broad at the supra-median cell, 6–7-septate, ends asymmetrical, not constricted at any septum, hyaline, smooth walled. Asexual morph (in culture): Hyphomycetous, chlamydosporous. Chlamydospores 50–135 μm long, 30–80 μm wide (x̄ = 85 × 55 μm, n = 50), holoblastic, sometimes intercalary, broadly oval to ellipsoid, dictyoseptate, pale brown when immature, darkened to black when matured, with terminal cells round and pale brown.

Culture characteristics: Conidia germinating on water agar and producing germ tubes within 36 h. Colonies growing slowly on malt extract agar (MEA), circular, with flat surface, veined and without wrinkle, edge entire, reaching 21 mm in 6 weeks at 28 °C, brown to dark brown in MEA media. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown, becoming dark brown due to the development of chlamydospores.

Material examined: THAILAND, Chiang Rai, Muang, Ban Nang Lae Nai, on submerged decaying wood in a freshwater stream, 28 November 2015, Saranyaphat Boonmee and Yong-Zhong Lu, TUB05–1 (MFLU 17–1167 = HKAS 100723), living culture, MFLUCC 16–0025; Ibid., TUB03–4 (MFLU 17–1170), living culture, MFLUCC 16–1129; THAILAND, Krabi, Muang, Khao Khram, Ban Nong Jig, Tha Pom Klong Song Nam, on submerged decaying wood in freshwater stream, 16 December 2015, Saranyaphat Boonmee, TP01–5 (MFLU 17–1181), living culture, MFLUCC 16–1126.

Notes: Morphologically, our new collections found from decaying wood substrate have typical chlamydospores, which are similar to Chlamydotubeufia huaikangplaensis and Dictyospora thailandica (Brahamanage et al. 2017; Hyde et al. 2017). Phylogenetically, these three newly obtained isolates form a monotypic clade with two known Chlamydotubeufia khunkornensis isolates and can be recognized as one species. Furthermore, our phylogenetic result shows that this taxon shares a sister relationship to Neochlamydotubeufia fusiformis but distantly related to Chlamydotubeufia and Dictyospora members, therefore, we synonymize it as Neochlamydotubeufia khunkornensis. (Lu et al. 2018)

Freshwater distribution: Thailand (Lu et al. 2018)