Tubeufiales » Tubeufiaceae » Neotubeufia

Neotubeufia krabiensis

Neotubeufia krabiensis Chaiwan, Boonmee, Y.Z. Lu & Hyde

Index Fungorum number: IF553872; Facesoffungi number: FoF03778

Etymology: From the Latin –ensis, meaning associated with and Krabi in reference to the type locality of Krabi Province.

Holotype: MFLU17–1184

 

Saprobic on submerged decaying wood. Sexual morph: Ascomata 340–390 μm high × 365–400 μm diam, superficial, solitary, scattered, subglobose dark-brown to black, lacking setae. Peridium 58–68 μm wide, comprising several layers brown to dark brown cells of textura angularis, outer layer darkened cells and inner layer pale brown to hyaline cells. Hamathecium comprising numerous filiform, septate, hyaline pseudoparaphyses. Asci 120–145 × 12–15 μm ( = 132 × 14 μm, n = 20), 8-spored, bitunicate, cylindrical, apically rounded, long pedicellate or sessile. Ascospores 55–75 × 5–6.5 μm ( = 66 × 6 μm, n = 20), fasciculate, cylindrical to cylindric-fusiform, elongated, slightly curved, tapering towards rounded ends, 6–7-septate, hyaline, guttulate, smooth-walled. Asexual morph: Undetermined.

 

Culture characteristics: Ascospores germinating on water agar and producing germ tubes within 12 hours. Colonies growing on MEA, circular, umbonate, rough and wrinkled at surface, edge entire, reaching 12 mm in 2 weeks at 28 °C, pale brown to brown, mycelium superficial and partially immersed, branched, septate, hyaline to pale brown, smooth.

 

Material examined: THAILAND, Krabi, Muang, on decaying wood in freshwater stream, 16 December 2015, Saranyaphat Boonmee, TP01–3 (MFLU 17–1184, holotype; GZAAS 17–0017 paratype), ex-type living culture, MFLUCC 16–1125.

 

Notes: Neotubeufia krabiensis found in a freshwater habitat and described as a new species, shares some similar morphological characters with Tubeufia species in that both have bitunicate, cylindrical asci and fasciculate, cylindrical to long cylindric-fusiform, elongate, slightly curved, phragmoseptate ascospores. However, phylogenetic analysis placed N. krabiensis in a separate clade from Tubeufia group with strong support. Therefore, N. krabiensis is clearly distinct taxon from Tubeufia. Neotubeufia differs from the type species T. javanica and other species in Tubeufia by its dark ascomata and cylindrical to cylindric-fusiform, multi-septate with distinctively guttulate ascospores. (Notes from Chaiwan et al. 2017)

 

Freshwater distribution: Thailand (Chaiwan et al. 2017)