Tubeufiales » Tubeufiaceae » Tubeufia

Tubeufia chlamydospora

Tubeufia chlamydospora Y.Z. Lu, Boonmee & K.D. Hyde

Index Fungorum number: IF 554899; Facesoffungi number: FoF 04754

Etymology: “chlamydospora” referring to chlamydospores of this fungus.

Holotype: MFLU 17–1172

 

Saprobic on submerged decaying wood in a freshwater stream. Sexual morph Undetermined. Asexual morph Hyphomycetous, chlamydosporous. Conidiophores lacking. Conidiogenous cells holoblastic, monoblastic, integrated, cylindric, apical, pale brown. Chlamydospores acrogenous, carbonaceous, friable, solitary, variable in shape, globose or subglobose, ovoid to irregular, indistinctly dictyoseptate, verrucose, 65–100 μm long, 60–90 μm wide ( = 83 × 71 μm, n = 20), hyaline to pale brown when young, dark brown to black when matured. Some conidia formed small globose to subglobose secondary conidia (Fig. 1n, o), 8–12 μm in diam., attached to the margin of the chlamydospore, pale brown. (Lu et al. 2018)

 

Culture characteristics: Conidia germinating on water agar and producing germ tubes within 24 h. Colonies growing on malt extract agar, circular, with flat surface, edge filiform, reaching 26 mm in 4 weeks at 28 °C, brown to dark brown in MEA media. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown.

 

Material examined: THAILAND, Krabi, Muang, Nongtalay, Khao Hang Nak, on submerged decaying wood in a freshwater stream, 16 December 2015, Saranyaphat Boonmee, HN05–2 (MFLU 17–1172, holotype), ex-type living cultures, MFLUCC 16–0223, TBRC 8896.

 

Notes: Morphologically, Tubeufia chlamydospora resembles Artocarpomyces paradoxus in having conidia that are variable in shape, globose or subglobose, ovoid to irregular, indistinctly dictyoseptate, verrucose, but can be distinguished by its larger conidial size (65–100 × 60–90 μm vs. 55–90 × 30–60 μm) and different conidiophore morphology as well as lack of stroma (Subramanian 1994). Tubeufia chlamydospora resembles Chlamydotubeufia huaikangplaensis and Neochlamydotubeufia khunkornensis in colony features on decaying woody substrate but can be distinguished by conidial morphology. The conidia of T. chlamydospora are acrogenous, sometimes with small globose to subglobose secondary conidia, while C. huaikangplaensis and C. khunkornensis are developed from hyphae having a pedicellate-like mycelium exist in both ends. Besides, the conidia of T. chlamydospora are vaguely dictyoseptate, while C. huaikangplaensis and C. khunkornensis have clearly dictyoseptate conidia. Phylogenetically, T. chlamydospora groups within Tubeufia and shares a sister relationship to T. machaerinae with high bootstrap support (Lu et al. 2018). The close phylogenetic relationship between T. chlamydospora and T. machaerinae is unexpected as their morphology is remarkably different, but supported by strong molecular evidence, therefore, we introduce the new collection as a new Tubeufia species. (Lu et al. 2018)

 

Freshwater distribution: Thailand (Lu et al. 2018)