Tubeufia claspisphaeria Kodsueb
Index Fungorum number: IF 488559
Etymology: In reference to the clasp-like setae on the ascomata.
Holotype: HKU (M) 17123
Saprobic on submerged decaying wood in a freshwater habitat. Sexual morph: Ascomata 185–330(350) µm diam, superficial, globose, solitary or grouped, hyaline to pale brown when immature, dark brown becoming black when mature, membranous, ostiolate, with setae that form around ostiole. Setae from surface of ascomata, up to 47 µm long and 10 µm wide, (0–)1(–2)-septate, dark brown, thick-walled, hook-shaped. Peridium up to 60 µm wide, vinaceous brown, comprising 4–5 layers of thick-walled textura globulosa, outer cells brown-walled, inner cells with hyaline walls. Pseudoparaphyses 1.5–2.5 µm wide, hypha-like, numerous, cellular, unbranched. Asci (93–)105 120(–127) × (12–)14–16(–18) µm (x̄ = 5 109 × 15.1 µm, n = 15), 8-spored, cylindric-clavate, bitunicate, with a small ocular chamber, persistently pedicellate. Ascospores (34–)45–60(–64) × 5–7(–8) µm (x̄ = 5 49 × 5.9 µm, n = 35), fasciculate, elongate-fusiform, (3–)5–6(–8)- septate, hyaline, sometimes slightly curved, smoothwalled, guttulate, lacking appendages or sheaths. Asexual morph Undetermined.
Cultural characteristics: Colonies on potato-dextrose agar dark green to black, 1 cm diam in 1 wk at room temperature (~28oC). Mycelium mostly immersed, aerial mycelium velvety to fluffy, no pigment diffusing into agar, not sporulating even after submergence overnight in a bubble chamber. Mycelium less dense at the outer edge than in center, with branching mycelial strands extending from edge of colony.
Material examined: CHINA. Hong Kong, Lantau Island, Trappist Monastery, small stream in forest, on wood partially submerged, 21 Apr 2002, K.D. Hyde (HKU[M] 17123). Living cultures ex holotype HKUCC 9116, HKUCC 9119.
Freshwater distribution: Hong Kong, China (Kodsueb et al. 2004)
Figs 7–16. Tubeufia claspisphaeria (HKU (M) 17123, holotype). 7. Ascomata on natural substratum. 8. Longitudinal section through an ascoma. 9. Setae. 10. A cluster of mature and immature asci. 11. Asci. 12–16. Ascospores. Pseudoparaphyses in Fig. 10 (arrowed). Scale bars: 7 = 250 µm, 8 = 50 µm, 9 = 25 µm, 10–11 = 30 µm, 12 = 15 µm, 13–16 = 10 µm. Figs 8–10 and 12 mounted in water, other figures mounted in lactophenol. (Kodsueb et al. 2004)
Kodsueb R, Lumyong S, Lumyong P, McKenzie EHC, Ho WH, Hyde KD (2004) Acanthostigma and Tubeufia species, including T. claspisphaeria sp. nov., from submerged wood in Hong Kong. Mycologia 96:667–674. https://doi.org/10.1080/15572536.2005.11832963