Tubeufia cylindrothecia
Tubeufia cylindrothecia (Seaver) Höhn.
Basionym: Ophionectria cylindrothecia Seaver, Mycologia 1(2): 70 (1909)
Index Fungorum number: IF 340543
Saprobic on decaying wood in terrestrial and freshwater habitats. Sexual morph: Undetermined. Asexual morph: Colonies superficial, effuse, gregarious, white to pale brown. Mycelium composed of partly immersed, hyaline to pale brown, septate, sparsely branched hyphae, with masses of conidia. Conidiophores 60–140 × 3–5 μm (x̄ = 108 × 3.8 µm, n = 10), macronematous, mononematous, cylindrical, septate, branched or unbranched, erect, flexuous, pale brown to brown, smooth-walled. Conidiogenous cells 10.5–18 × 3.5–4 μm (x̄ = 15.5 × 3.8 µm, n = 5), holoblastic, mono- to poly-blastic, integrated, terminal, smooth, truncate at the apex after conidial secession, each with single or several conidia. Conidia solitary, acrogenous, helicoid, rounded at tip, 24.5–28 μm diam. and conidial filament 3–4.2 μm wide (x̄ = 26 × 3.6 μm, n = 10), 100–210 μm long, coiled 2.75–3.25 times, indistinctly multi-septate, guttulate, hyaline to pale brown, smooth-walled. (Dong et al. 2020)
Culture characteristics: On PDA, colony irregular, dark brown to black from above and below, surface rough, with dense superficial or immersed mycelium, most mycelium immersed in medium, dry, edge undulate.
Material examined: THAILAND, Chiang Mai Province, on submerged wood in a stream, 29 November 2010, H. Zhang, d6 (MFLU 11-0969), living culture MFLUCC 10-0919; ibid., d29 (MFLU 11-1107), living culture MFLUCC 11-0076.
Notes: Our two new collections MFLUCC 10-0919 and MFLUCC 11-0076 are identified as Tubeufia cylindrothecia based on the identical ITS and LSU sequence data to the ex-type strain of T. cylindrothecia, and phylogenetic analysis (Dong et al. 2020). The asexual morph of T. cylindrothecia was first reported by Luo et al. (2017) and later encountered by Lu et al. (2018) from freshwater habitats. Our freshwater collections have longer conidiophores (60–140 μm vs. 50–81 μm) and shorter conidia (100–210 μm vs. 256–314 μm) than the holotype (Luo et al. 2017). Additionally, we found polyblastic conidiogenous cells in MFLU 11-0969, while they are only monoblastic in the holotype (Luo et al. 2017).
Freshwater distribution: China (Luo et al. 2017), Thailand (Lu et al. 2018; Dong et al. 2020)
Fig 1 Tubeufia cylindrothecia (MFLU 11-0969). a Colonies on submerged wood. b–e Conidiophores, conidiogenous cells and conidia. f–h conidia. i Germinated conidium. j, k Colony on PDA (left-front, right-reverse). Scale bars: b, i = 20 μm, c–h = 10 μm (Dong et al. 2020)
Fig 2 Tubeufia cylindrothecia (MFLU 11-1107). a Colonies on submerged wood. b–d Conidiophores, conidiogenous cells, conidia. e, f conidia. g Germinated conidium. h, i Colony on PDA (up-front, down-reverse). Scale bars: b, e–g = 20 μm, c = 5 μm, d = 10 μm (Dong et al. 2020)
References
Dong W, Wang B, Hyde KD, McKenzie EHC, Raja HA, Tanaka K, Abdel-Wahab MA, Abdel-Aziz FA, Doilom M, Phookamsak R, Hongsanan S, Wanasinghe DN, Yu X-D, Wang G-N, Yang H, Yang J, Thambugala KM, Tian Q, Luo Z-L, Yang J-B, Miller AN, Fournier J, Boonmee S, Hu D-M, Nalumpang S, Zhang H (2020) Freshwater Dothideomycetes. Fungal Divers 105:319–575. https://doi.org/10.1007/s13225-020-00463-5
Lu YZ, Liu JK (Jack), Hyde KD, Jeewon R, Kang JC, Fan C, Boonmee S, Bhat DJ, Luo ZL, Lin CG, Eungwanichayapant PD (2018) A taxonomic reassessment of Tubeufiales based on multi-locus phylogeny and morphology. Fungal Divers 92:131–344. https://doi.org/10.1007/s13225-018-0411-y
Luo ZL, Bhat DJ, Jeewon R, Boonmee S, Bao DF, Zhao YC, Chai HM, Su HY, Su XJ, Hyde KD (2017) Molecular phylogeny and morphological characterization of asexual fungi (Tubeufiaceae) from freshwater habitats in Yunnan, China. Cryptogam Mycol 38:27–53. https://doi.org/10.7872/crym/v38.iss1.2017.27
Recent Genus
1Neochlamydotubeufia
Neohelicomyces
Recent Species
Neohelicomyces submersusNeohelicomyces hyalosporus
Neohelicomyces grandisporus