Tubeufiales » Tubeufiaceae » Tubeufia

Tubeufia latispora

Tubeufia latispora Y.Z. Lu, Boonmee & K.D. Hyde

Index Fungorum number: IF 818985; Facesoffungi number: FoF 02691

Etymology: ‘latispora’ referring to wide ascospores.

Holotype: MFLU 16-2658

 

Saprobic on decaying wood in a stream. Sexual morph: Ascomata 267–331 μm high × 166–215 μm diam. (x̄ = 287 × 185 μm, n = 20), superficial, gregarious, ellipsoidal, globose to subglobose, dark brown. Setae (99–)135–317 × 3–6.5 μm (x̄ = 229 × 4.5 μm, n = 30), dense, flexuous, covering the whole ascoma, unbranched, rounded at apical end, septate, dark brown. Peridium 24–44 μm wide, composed cells of textura angularis, dark, with innermost part comprising thin layers of hyaline to pale brown cells of textura subprismatica. Hamathecium comprising numerous filiform, septate, branched, hyaline pseudoparaphyses. Asci 137–235 × 20–30 μm (x̄ = 181 × 24 μm, n = 30), 8-spored, bitunicate, cylindrical, rounded at the apex, with an ocular chamber, thick-walled, sessile. Ascospores 68–105 × 7–10 μm (x̄ = 83 × 8.5 μm, n = 50), fasciculate, broad fusiform, cylindrical to long subfusiform, elongate, slightly curved, tapering towards the rounded ends, 16–21-septate, hyaline, smooth-walled. Asexual morph: Undetermined. (Lu et al. 2017)

 

Culture characteristics: Ascospores germinating in water agar (WA) within 24 h and germ tubes produced from a lower cell of ascospores. Colonies growing slowly on malt extract agar (MEA), irregular, with rough surface, edge undulate, reaching 10 mm in 2 weeks at 28 °C, initially brown and becoming dark brown when aged. Mycelium superficial and partly immersed, branched, septate, hyaline to pale brown, smooth-walled.

 

Material examined: THAILAND, Chiang Rai, Muang, Ban Nang Lae Nai, on decaying wood in a freshwater stream, 28 November 2015, Saranyaphat Boonmee and Yong-Zhong Lu, TUB06 (MFLU 16-2658, holotype; GZAAS 16-0140, isotype); ex-type living culture, MFLUCC 16-0027 = GZCC 16-0103.

 

Notes: Tubeufia latispora is introduced here as a novel species based on its distinct morphological and phylogenetic placement. The phylogenetic analysis recognises T. latispora belonging to the genus Tubeufia, where it is sister to T. longiseta with high support (100% BS and 1.00 PP). Tubeufia latispora formed a sexual morph on the natural substrate, which is morphologically quite similar to T. longiseta (Dai et al. 2017). Although both species have similar coloured and sized ascomata and are covered by dense, flexuous setae, T. latispora differs from T. longiseta in the sizes of asci and ascospores. The asci (137–235 × 20–30 μm) of T. latispora are longer and wider than T. longiseta (90–140 × 14.5–18 μm) and ascospores (68–105 × 7–10 μm) are longer and wider than T. longiseta (38.5–55.5 × 4.5–6 μm) as well. Therefore, we introduced it as a novel species. (Lu et al. 2017)

 

Freshwater distribution: Thailand (Lu et al. 2017)

 

Fig 1 Tubeufia latispora (MFLU 16-2658, holotype). a Superficial ascomata on substrate. Note ascomata surrounded by dark brown setae. b Ascoma. c Peridium. d Seta. e Hamathecium. f–h Asci. i Germinating ascospore. j–l Ascospores. Scale bars: a = 500 μm, b = 100 μm, c–d, f–i = 50 μm, e, j–l = 20 μm (Lu et al. 2017)

 

References

Lu YZ, Boonmee S, Dai DQ, Liu JK, Hyde KD, Bhat DJ, Ariyawansa H, Kang JC (2017) Four new species of Tubeufia (Tubeufiaceae, Tubeufiales) from Thailand. Mycol Prog 16:403–417. https://doi.org/10.1007/s11557-017-1280-6

Dai DQ, Phookamsak R, Wijayawardene NN, Li WJ, Bhat DJ, Xu JC, Taylor JE, Hyde KD, Chukeatirote E (2017) Bambusicolous fungi. Fungal Divers 82:1–105. doi:10.1007/s13225-016-0367-8

 

Recent Genus

1
Neochlamydotubeufia
Neohelicomyces

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